4-[fluoro(dimethyl)silyl]butanenitrile

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 25 - November 16, 2016

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

Adopted 17 th July, 1992

Deviations:

no

GLP compliance:

no

Remarks:

The study was perfonned according to the study plan and SOPs, which was in compliance with the "Guidelines of Chemical Testing Good Laboratory Practices" (Ministry of Environmental Protection of the People's Republic of China).

Test material

Test material information

Constituent 1

Reference substance name:

4-[fluoro(dimethyl)silyl]butanenitrile

EC Number:

812-925-4

Cas Number:

1639345-42-8

Molecular formula:

C6H12FNSi

IUPAC Name:

4-[fluoro(dimethyl)silyl]butanenitrile

Test material form:

liquid

Details on test material:

- Purity test date: 97.7% Silane, 3-cyanopropyl dimethyl fluoro; 1-1.4% Silane, 2-cyanopropyl dimethyl fluoro
- Lot/batch No.: SO408
- Expiration date of the lot/batch: November 2016

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Aeration tank of Liede Sewage Treatment Plant of Guangzhou
- Treatment: The sludge was removed any coarse particles and impurities on the surface, then was washed with the test medium (1 lOOg, lOmin, repeated for 3 times), the supernatant liquid phase was decanted and the solids was re-suspended in the test medium. The sludge suspension was detennined as 8.3g/L. Kept it aeration until required. When testing, the inoculum was diluted to 4.0g/L and added to each test vessel to give a final concentration of 30mg suspended solids per liter.
- Determination of cell number: The total number of cell in the test system was detennined as 4.0x10E7 cfu/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

75 mg/L

Based on:

act. ingr.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
All of the test bottles were stirred and incubated on OxiTop® Control BOD Test System at 22°C± 1°C in the dark and closed condition over 28 days.

CONTROL AND BLANK SYSTEM
Bottles 1, 2 & A (test suspension) Containing test substance, inoculum and test medium
Bottles 3, 4& B (inoculum blank) Containing inoculum and test medium
Bottle 5 (procedure control) Containing reference substance, inoculum and test medium
Bottle 6 (toxicity control) Containing test substance, reference substance, inoculum and test medium
Note: Bottles A & 8 for determination of nitrite and nitrate concentrations at the start of the test.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

43.9

Sampling time:

28 d

Remarks on result:

other: mean of two replicates

Details on results:

Determination of the nitrite and nitrate concentrations of bottles A and B at the start of the test: As the results showed that the test substance cannot be considered to be readily biodegradable, the nitrite and nitrate concentrations of bottles 1, 2, 3, 4 were not determined at the end of the test.

BOD5 / COD results

Results with reference substance:

97.7% after 14 d. Thus, the 60% pass level was reached.

Any other information on results incl. tables

The toxicity control attained 50.5% degradation after 14 d. Thus, the test substance is not inhibitory to the inoculum.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The percentage biodegradation of the test substance was 43.9% at the end of the test under the conditions of the study, so the test substance cannot be considered to be readily biodegradable.