Reaction mass of Octyl-3-[3-tert-butyl-4-hydroxy-5-(5-chloro-2H-benzotriazole-2-yl)phenyl]propionate and 2-Ethylhexyl-3-[3-tert-butyl-4-hydroxy-5-(5-chloro-2H-benzotriazole-2-yl)phenyl]propionate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From May 13, 2002 to June 21, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: A clone of this species was originally supplied by the University of Sheffield/UK in 1992, defined from the supplier as clone 5. Since this date, the clone is bred in the laboratories of RCC.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250.0 mg/L as CaCO3

Test temperature:

20 °C

Nominal and measured concentrations:

A water accommodated fraction (WAF) of the test item with a loading rate of 100 mg/L.

Details on test conditions:

- Test volume: 50 mL
- Aeration: None
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Photoperiod: 16-hr light: 8-hr dark

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Table 1. Influence of TK 12871 (TINUVIN 109) on the mobility of Daphnia magna

Loading rate (mg/L)	No. of daphnids tested	Immobilized daphnids after 24 hours		Immobilized daphnids after 48 hours
		No.	%	No.	%
Control	20	2	10*	2	10*
100	20	0	0	0	0

* An immobilization rate of 10% is tolerated by the test guidelines.

 

Table 2. Appearance of the test medium during the test period

Loading rate (mg/L)	Exposure time
	0 hours	24 hours	48 hours
100	0	0	0

Abbreviations:

0: no remarkable observations, clear test medium

1: homogeneous dispersion in the water, turbidity observable

2: noticeable turbidity caused by the test item

3: noticeable coloration caused by the test item

4: Inhomogeneous dispersion of the test item

5: precipitation of the test item

6: test item at the surface

7: test item lying at the bottom of the test beaker

 

Table 3. Dissolved oxygen concentrations, pH values and temperature in the test medium and the control

Loading rate (mg/L)	Start (0 hours)			End (48 hours)
	pH	Oxygen (mg/L)	Temperature (°C)	pH	Oxygen (mg/L)	Temperature (°C)
Control	8.0	8.7	20	7.7	8.9	20
100	7.9	8.5	20	7.8	8.7	20

Validity criteria fulfilled:

yes

Conclusions:

According to EU Method C.2 and OECD 202 test method, the 48-hr EC0 of TK 12871 (TINUVIN 109) was at least at the loading rates of 100 mg/L.

Executive summary:

This test using the procedures outlined in the RCC Study Plan for 841658, EU Method C.2 (1992) and OECD 202 (OECD, 1984). The results of this test for TK 12871 (TINUVIN 109) show that all validity criteria were met. All results are based on the loading rate of the test item of 100 mg/L. The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours) and the 48-hour EC0 of TK 12871 (TINUVIN 109) to Daphnia magna were determined to be at least at the loading rate of 100 mg/L. The 48-hour NOEC and the 48-hour EC0 might even be higher but loading rates in excess of 100 mg/L were not tested. The 48-hour EC50 and the 48-hour EC100 were clearly higher than the loading rate of 100 mg/L. These values could not be quantified since the test item had no toxic effect on the daphnids up to the highest test item concentration which could be dissolved in the test water under the conditions of the test.

Description of key information

The 48-hr EC0 of TK 12871 (TINUVIN 109) was at least at the loading rates of 100 mg/L for Daphnia magna (OECD TG202, EU Method C.2).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In limit test, not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration of control and test groups at the end of the test was ≥3 mg O₂/L. All test validity criteria were met.The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. All results are based on the loading rate of the test item of 100 mg/L. The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours) and the 48-hour EC0 of TK 12871 (TINUVIN 109) to Daphnia magna were determined to be at least at the loading rate of 100 mg/L. The 48-hour NOEC and the 48-hour EC0 might even be higher but loading rates in excess of 100 mg/L were not tested. The 48-hour EC50 and the 48-hour EC100 were clearly higher than the loading rate of 100 mg/L. These values could not be quantified since the test item had no toxic effect on the daphnids up to the highest test item concentration which could be dissolved in the test water under the conditions of the test.