Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-044-6 | CAS number: 131-92-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 August 1981 and were completed on 21 September 1981.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- ETAD Toxicological Method No 005
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N'-(10,15,16,17-tetrahydro-5,10,15,17-tetraoxo-5H-dinaphtho[2,3-a:2',3'-i]carbazole-4,9-diyl)bis(benzamide)
- EC Number:
- 205-044-6
- EC Name:
- N,N'-(10,15,16,17-tetrahydro-5,10,15,17-tetraoxo-5H-dinaphtho[2,3-a:2',3'-i]carbazole-4,9-diyl)bis(benzamide)
- Cas Number:
- 131-92-0
- Molecular formula:
- C42H23N3O6
- IUPAC Name:
- N,N'-(10,15,16,17-tetrahydro-5,10,15,17-tetraoxo-5H-dinaphtho[2,3-a:2',3'-i]carbazole-4,9-diyl)bis(benzamide)
- Test material form:
- solid: particulate/powder
- Details on test material:
- Vat Brown 3
Constituent 1
- Specific details on test material used for the study:
- Reference no: Y01776/010/001
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-induced rat liver S9
- Test concentrations with justification for top dose:
- 4 to 2500 µg/plate
Limit of solubility: 2000 µg/plate - Vehicle / solvent:
- Dimethylsulphoxide (DMSO)
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethylsulphoxide (DMSO)
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- other: 2-Aminoanthracene, Daunorubicin, N-Methyl-N'-nitro-N-nitrosoguanidine
- Details on test system and experimental conditions:
- The Salmonella/microsome mutagenicity assay was conducted using the plate incorporation assay as described by Ames et al (1975), with certain modifications as specified in ETAD Toxicological Method No 005
The test compound was assayed once, over a dose range of 2500-4.0 µg/plate, with and without metabolic activation. If the test compound gave positive or inconclusive results in this initial assay were retested using the appropriate strains and metabolic activation conditions. Where appropriate, these repeat assays were performed over different dose ranges to allow a more detailed examination of the responses originally observed. Due to the precipitation of the test compound at high plate concentrations, samples which gave an unequivocal negative result in the initial assay were not retested at higher dose levels. The studies commenced on 11 August 1981 and were completed on 21 September 1981. - Evaluation criteria:
- A positive response in the assay system was taken to be a two-fold or greater increase in the mean number of revertant colonies appearing in the test plates over and above the background spontaneous reversion rate observed with the solvent, together with evidence of a dose response at sub-toxic concentrations of the test substance. Observed positive responses were validated by replica plating (Elek and.Hilson, 1954). This technique verifies whether true reversion to prototrophy has occurred.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The test compound, together with appropriate positive and negative controls, was examined in at least two independent experiments both in the presence and absence of an auxiliary metabolising system (S9).
In each experiment, the positive controls responded as expected indicating that the assay was performing satisfactorily. Under the conditions of this assay, Substance H112339 gave a negative, ie non-mutagenic response in S-typhimurium strains TA1535, TA1537, TA98 and TA100 and in E.coli strains WP2P and WP2P uvrA in both the presence and absence of S9.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this assay, the test substance gave a negative, is non-mutagenic, response with S-typhimurium strains TA1535, TA1537, TA98 and TA100 in the presence and absence of an auxiliary metabolising system (S9).
- Executive summary:
When tested to the limit of solubility (2000 µg/plate), this compound gave a negative result in the absence of metabolic activation. In the presence of S9, a positive result was obtained in strain TA1537 at a single dose (500 µg/plate), with no dose-related response at lower plate concentrations. On retesting, this result was not repeatable, and would thus appear to be an artifact rather than a true positive result. The compound gave a negative result with the other three strains in the presence of S9.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.