6,10-dimethylundecan-2-one

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundecan-2-one

EC Number:

216-509-8

EC Name:

6,10-dimethylundecan-2-one

Cas Number:

1604-34-8

Molecular formula:

C13H26O

IUPAC Name:

6,10-dimethylundecan-2-one

Details on test material:

- Name of test material (as cited in study report): Tetrahydrogeranylaceton
- Lot/batch No.: B 633
- Analytical purity: 97.5 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was added in the required amount to the test vessel with about 180 ml deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. Then synthetic medium was added, the pH-value was adjusted to 7,5 ± 0,5, the inoculum was added and the test started.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge from laboratory wastewater plant treating municipal sewage. Concentration of dry substance 1 g/I.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

20 °C

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
1,
10,
100

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Fill volume: 250 ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: :8 ml/vessel 1 00-fold concentrated OECD

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC20: ca. 2.4 mg/L
EC50: ca. 50 mg/L