Registration Dossier
Registration Dossier
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EC number: 221-909-0 | CAS number: 3278-22-6
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,1'-[methylenebis(sulphonyl)]diethylene
- EC Number:
- 221-909-0
- EC Name:
- 1,1'-[methylenebis(sulphonyl)]diethylene
- Cas Number:
- 3278-22-6
- Molecular formula:
- C5H8O4S2
- IUPAC Name:
- (ethenesulfonyl)methanesulfonylethene
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- Preliminary toxicity determination: 1, 3.3, 10, 33.3, 100, 333, 1000, 3330, 5000 microgram/plate
Mutagenic reponse test without S9-mix: 0.3, 1, 3.3, 10, 20 microgram/plate
Mutagenic reponse test with S9-mix: 0.3, 1, 3.3, 10, 33.3 microgram/plate
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- not specified
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- V190110 is considered not mutagenic in this test system and under present experimental conditions.
- Executive summary:
V190110 was tested in the Ames Salmonella/microsome plate test up to 20 microgram/plate in the absence of S9-mix and up to 33.3 microgram/plate in the presence of S9-mix. The test substance did not induce a dose-related increase in the number of revertant (His+) colonies in both tester strains (TA98 and T100).
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