Registration Dossier
Registration Dossier
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EC number: 946-798-2 | CAS number: -
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
For the Inhibition of growth rate, the 7-day ErL10 and ErL50 was determined to be > 200 mg/L (nominal). The 7-day NOEL was established to be 200 mg/L (nominal).
Key value for chemical safety assessment
Additional information
The effects of a Water Soluble Fraction (WSF) of the test item on the growth of the monocotyledonous aquatic plant species Lemna gibba was determined according to OECD Guideline 221 and in compliance with GLP principles (BASF SE, 2017). Since the test item is poorly water soluble, a modification of the standard method for the preparation of aqueous media was performed and a WSF in accordance with ECETOC 1996, OECD Series No. 23 (2000) prepared. Furthermore, as the test item contains a large proportion of unidentified by-components (UVCB substance), it is treated as a mixture and the results are based on nominal loadings rather than on mean measured concentrations. Lemna gibba was exposed to the test item for 7 days under semi-static conditions with daily renewal. Based on a preliminary test, 6 nominal test item concentrations were tested in a geometrical series with a dilution factor of 2nd root of 10: 0.632, 2.00, 6.32, 20.0, 63.2 and 200 mg/L. Three replicates were investigated for each test concentration and six for the control. Frond numbers were assessed on days 0, 2, 5 and 7. Environmental parameters (light, pH and temperature) were within the acceptable limits. The measured concentrations (analyzed via LC-MS/MS) of the test item in fresh media on days 0, 1 and 6 in the highest tested loading were in the range of 0.0209 to 0.0578 mg test item/L. As a result, the 7-day ErL10, ErL20 and ErL50 as well as the LOEL for the growth rate Inhibition was determined to be > 200 mg/L. The 7-day NOEL (growth rate) was established to be 200 mg/L. For the inhibition of yield, the 7-day EyL2, EyL50 and LOEL was determined to be > 200 mg/L. The 7-day EyL10 and NOEL (inhibition of yield) was established to be 71.8 (15.6 - > 200) mg/L and 200 mg/L, respectively. All validity criteria of the test guideline were fulfilled.
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