4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
1.To evaluate short term toxicity of test material on fish
2.To evaluate the acute toxicity of test material on Fathead Minnow fish.

GLP compliance:

not specified

Details on sampling:

2. composite stored a t 20 C in dark.Stock solution refrigerated

Vehicle:

not specified

Test organisms (species):

other: 1.Bruchydunio rerio , 2.Pimephales promelas

Details on test organisms:

2.TEST ORGANISM
- Common name: Fathead Minnow
- Source: Berry water gardens , windmill fish hatcheries , North Carolina
- Length at study initiation : 5.2 cm
- Weight at study initiation: 1.3 g
- Feeding during test: starved 48 hrs before test

ACCLIMATION
- Acclimation period: 10 days
- Acclimation conditions : maintained in plastic stock tank in continuously running charcoal –sand filtered Chapel Hill tap water
- Type and amount of food: protein enriched fish food
- Feeding frequency: 5-7 times per week

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

2) 32mg/l

Test temperature:

2)15°C

pH:

2)6.8-8.5

Nominal and measured concentrations:

2)0, 0.1, 1.8, 3.2, and 5.6, 10.0 mg/l

Details on test conditions:

2. Test vessel: 5-gallon wide mouth glass jar
- No. of organisms per vessel: 10

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Read across 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Read across 2

Details on results:

2. Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic to fish atleast in the concentration range 12-100 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.Test material was used to evaluate the short term toxicity onBruchydunio rerio. The effect concentration (EC50 was considered to be less the 100 mg/l.

2.Test material was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

 

10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 12 mg/l . Toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >12 mg/l

Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 12 -100 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for short term toxicity of fish as " acute Aquatic 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the concentration range 12 -100mg/L .

Description of key information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.Test material was used to evaluate the short term toxicity onBruchydunio rerio. The effect concentration (EC50 was considered to be less the 100 mg/l.

2.Test material was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

 

10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 12 mg/l . Toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >12 mg/l

Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 12 -100 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for short term toxicity of fish as " acute Aquatic 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the concentration range 12 -100mg/L .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.Test material was used to evaluate the short term toxicity onBruchydunio rerio. The effect concentration (EC50 was considered to be less the 100 mg/l.

2.Test material was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

 

10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 12 mg/l . Toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >12 mg/l

Before death, fish tended to become very slow moving at higher concentration. Dead fish were dyed on heads and fins . Surviving fish were dyed dark green in concentration above 1mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 12 -100 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for short term toxicity of fish as " acute Aquatic 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the concentration range 12 -100mg/L .