L-Glutamic acid, N-coco acyl derivs., compds. with triethanolamine (1:1)

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 August 2017 to 12 August 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Key result

Water solubility:

>= 383 000 mg/L

Conc. based on:

test mat.

Incubation duration:

72 h

Temp.:

20 °C

pH:

4.6

Details on results:

RESULTS OF THE SHAKE FLASK METHOD
- pH of the samples varied in the range of 4.60 – 4.62.
- Considering that the large amount of the test material was dissolved in every case without insoluble residue and the test mixtures were homogeneous by visual assessment, the measured concentrations depend on the amount of added test material and can be handled individually. The RSD% of the amount of added test material was 1.1 %, the RSD% of the measured concentrations was 1.9 %.

Table 1: Results of the determination of test material concentrations

Measured concentration after 1 day (mg/mL)	pH	Measured concentration after 2 days (mg/mL)	pH	Measured concentration after 3 days (mg/mL)	pH	Solubility of the test material (mg/mL)
376	4.62	383	4.60	383	4.61	≥383
377	4.61	382	4.61	373	4.60
362	4.60	369	4.62	382	4.61

Conclusions:

Under the conditions of this study the water solubility of the test material was determined to be ≥ 383 mg/mL at 20 °C ± 0.5 °C using the shake-flask method.

Executive summary:

The water solubility of the test material was determined in accordance with the standardised guidelines OECD 105 and EU Method A.6., under GLP conditions.

The test material was dissolved in water at a temperature somewhat above the test temperature. When saturation was achieved the mixture was cooled and kept at the test temperature, as long as necessary to reach equilibrium. Subsequently, the mass concentration of the test material in the aqueous solution was determined by HPLC analysis. Before the HPLC analysis samples were diluted with acetonitrile – water 1:1 mixture.

Under the conditions of this study the water solubility of the test material was determined to be ≥ 383 mg/mL at 20 °C ± 0.5 °C using the shake-flask method.

Description of key information

Under the conditions of this study the water solubility of the test material was determined to be ≥ 383 mg/mL at 20 ± 0.5 °C using the shake-flask method.

Key value for chemical safety assessment

Water solubility:

383 000 mg/L

at the temperature of:

20 °C

Additional information

The water solubility of the test material was determined in accordance with the standardised guidelines OECD 105 and EU Method A.6., under GLP conditions. The study was awarded a reliability score of 1 in accordance with the criteria set forth by Klimisch et al. (1997).

The test material was dissolved in water at a temperature somewhat above the test temperature. When saturation was achieved the mixture was cooled and kept at the test temperature, as long as necessary to reach equilibrium. Subsequently, the mass concentration of the test material in the aqueous solution was determined by HPLC analysis. Before the HPLC analysis samples were diluted with acetonitrile – water 1:1 mixture.

Under the conditions of this study the water solubility of the test material was determined to be ≥ 383 mg/mL at 20 ± 0.5 °C using the shake-flask method.