Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-327-6 | CAS number: 6227-20-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 October 2016 to 14 December 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge : Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK,
- Storage conditions: on continuous aeration at a temperature of approximately 21 ºC
- Storage length: used the day of collection
- Preparation of inoculum for exposure: washed twice by settlement and resuspended in mineral medium
- Pretreatment: none
- Concentration of sludge: ss 3.0 g/L
- Water filtered: yes
- Type and size of filter used: 0.45 µm Gelman AcroCap filter - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20.7 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 10 mg/L
- Based on:
- other: mg C/L
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline
- Additional substrate: none
- Solubilising agent (type and concentration if used):none
- Test temperature: 22-24 °C
- pH: 7.7-7.8 pre-adjustment; 7.3-7.6 after adjustment
- Aeration of dilution water: CO2 free air at 30-100 mL/min
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 5 L culture vessels containing 3 L solution
- Number of culture flasks/concentration: inoculum control, procedure control and test substance in duplicate; toxicity control (single)
- Method used to create aerobic conditions: purging of CO2 free air and stirred continuously by magnetic stirrer
- Measuring equipment: TOC analyzer (Shimadzu TOC-VCPH TOC Analyzer)
- Test performed in closed vessels
- Details of trap for CO2 and volatile organics if used: NaOH (2 traps)
SAMPLING
- Sampling frequency: Days 0, 2, 6, 8, 10, 14, 21, 28 and 29 from first absober: days 0 and 29 from second absorber
- Sample storage before analysis: analysed immediately
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
STATISTICAL METHODS: NA - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- test for potential adsorption to filter matrices and/or the activated sewage sludge. 200 mg/L test substance was assessed for DOC in presence and absence of sludge with or without filtration through 0.45 µm Gelman AcroCap filter (4 treatments).
DOC concentration was 80-86% of nominal. No effect of filtration and or presence of sludge. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 29 d
- Remarks on result:
- other: after acidification with HCl
- Details on results:
- see table
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is not biodegradable under the conditions of the test.
- Executive summary:
In a CO2 evolution test (OECD 301B), the substance degradation was found to be 6% after 29 days. Therefore the substance is considered not readily biodegradable.
Reference
% Biodegradation
Day |
Procedure Control |
Test Item |
Toxicity Control |
0 |
0 |
0 |
0 |
2 |
47 |
2 |
25 |
6 |
63 |
4 |
37 |
8 |
64 |
8 |
39 |
10 |
66 |
8 |
42 |
14 |
70 |
11 |
43 |
21 |
63 |
12 |
41 |
28 |
62 |
6 |
41 |
29* |
72 |
6 |
39 |
*include any carry-over of CO2 detected in Absorber 2
Description of key information
In a CO2 evolution test (OECD 301B), the substance degradation was found to be 6% after 29 days. Therefore the substance is considered not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.