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EC number: 208-719-3 | CAS number: 539-48-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 1995-12-18 to 1995-12-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch No.: 51113
Purity: not specific - Analytical monitoring:
- not specified
- Details on sampling:
- Test item direct dispersion in reconstituted water
- Vehicle:
- no
- Details on test solutions:
- Definitive study concentration: following the initial range-finding study the concentrations of definitive study were: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L.
Preparation of definitive study solution: 25 mL of each of solutions a-d were added to each litre (final volume) of deionised water pH equal to 7.8 ± 0.2 adjusted (if necessary) with NaOH or HCI.
The reconstituted water was aerated until the dissolved oxygen concentration was approximately air-saturation value.
The reconstituted water has an approximate theoretical total hardness of 270 mg/L as CaCO3. - Test organisms (species):
- Daphnia magna
- Test type:
- not specified
- Water media type:
- other: reconstituted water
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 21 °C
- pH:
- 7.8 ± 0.2
- Nominal and measured concentrations:
- 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL containing 200 mL of test solution
- Aeration: No auxiliary aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Cumulative mortality (initial population = 10): 0 for 0.1, 1, 10 mg/L groups, 10 for 100 mg/L group. - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- EC50 at 24h: 24 mg/L, 95% Confidence limits is 18 to 32 mg/L
EC 50 at 48h: 16 mg/L, 95% Confidence limits is 14 to 19 mg/L
No Observed Effect Concentration (NOEC) at 24h: 18 mg/L
No Observed Effect Concentration (NOEC) at 48h: 10 mg/L
The No Observed Effect Concentration is based upon zero immobilization at this test concentration. - Validity criteria fulfilled:
- yes
- Conclusions:
- For test item, the EC 50 at 48h is16 mg/L.
- Executive summary:
The study was performed to assess the acute toxicity effect of test item to Daphnia magna according to the OECD Guideline for Testing of Chemicals No. 202 (1984).
For test item, EC50 at 24h is 24 mg/L, 95% Confidence limits is 18 to 32 mg/L; EC 50 at 48h is 16 mg/L, 95% Confidence limits is 14 to 19 mg/L; No Observed Effect Concentration (NOEC) at 24h is 18 mg/L; No Observed Effect Concentration (NOEC) at 48h is 10 mg/L. The No Observed Effect Concentration is based upon zero immobilization at this test concentration.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- See attached justification for read-across
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Referenceopen allclose all
Description of key information
One read-across study is available. For test item, the EC50 at 48h is 16 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 16 mg/L
Additional information
One read-across study is available which was conducted on Daphnia magna according to OECD Guideline 202.
For test item, EC50 at 24h is 24 mg/L, EC 50 at 48h is 16 mg/L, No Observed Effect Concentration (NOEC) at 24h is 18 mg/L, No Observed Effect Concentration (NOEC) at 48h is 10 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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