Butyl glycollate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 June 2019 - 11 October 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material:
120001168816
- Expiration date of the batch:
May 12, 2020
- Purity test date:
May 13, 2019

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Tightly closed in cool, dry, well ventilated area, refrigerator at 2-10°C

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Samples were taken from all test concentrations
- Sample storage conditions before analysis: Deep frozen (<= -18°C)

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea (Daphnia magna Straus)
- Strain/clone: Clone M10
- Age at study initiation: <24 hours :
- Source: inhouse culture, originally supplied by KU Leuven, Belgium
- Age of parental stock: > 35 days
- Feeding during test : Animals were not fed during the test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 mg/L CaCO3

Test temperature:

20.6 - 21.4°C

pH:

7.6 - 7.8

Dissolved oxygen:

98 - 104% of air saturation value

Conductivity:

652 µS

Nominal and measured concentrations:

Nominal: 4.27, 9.39, 20.7, 45.5 and 100 mg/L
Measured: 3.8, 8.4, 18.5, 40.6, and 89.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 100 mL glass vessels, covered with watch glasses; fill volume 50-60 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Test medium: Elendt M4
- Culture medium different from test medium: no
- Intervals of water quality measurement: Water quality parameters were measured at the start of the test, after temperature adaption of the test solutions, and at the end of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light: 8 hours dark
- Light intensity: 50–1000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobility, determined daily

RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 89.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities:
- Immobilisation of control: No immobile daphnids were observed in the control
- Effect concentrations exceeding solubility of substance in test medium: no

Validity criteria fulfilled:

yes

Conclusions:

A weak concentration-response relationship was observed with less than 50% immobility at the highest tested concentration. Thus, the EC50 was determined as higher than the highest test concentration of 100 mg/L (nominal). The test item concentrations were stable during the test with a mean recovery of 89.2%.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

A 48-hour acute toxicity study in Daphnia magna conducted under static conditions reported an EC₅₀value of >100 mg/l (nominal concentrations) based on mobility.