Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 229-858-6 | CAS number: 6789-99-7
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1971
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 4,5,6,7-tetrahydro-1H-benzotriazole
- EC Number:
- 229-858-6
- EC Name:
- 4,5,6,7-tetrahydro-1H-benzotriazole
- Cas Number:
- 6789-99-7
- Molecular formula:
- C6H9N3
- IUPAC Name:
- 4,5,6,7-tetrahydro-1H-1,2,3-benzotriazole
- Details on test material:
- coarse off-white powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: SD derived
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Geigy UK
- Age at study initiation: 5 weeks
- Weight at study initiation: 110g (females), 108g (males)
- Fasting period before study: 18 h
- Housing: single housing
- Diet: ad libitum
- Water: ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°F): 70+/-5
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Remarks:
- PEG 400
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 ml/kg - Doses:
- 500, 1000, 1500, 2000, 2500, 3000 mg/kg
- No. of animals per sex per dose:
- 5
- Statistics:
- From the mortality data recorded the LD50 value and its 95% confidence limits were calculated by the method of Litchfield, J.T and Wilcoxon, F. W., 1949, 3. Pharmac. Exp. Ther., 96, 99.
Results and discussion
- Preliminary study:
- In order to determine approximate dose levels for the main study, a range-finding study was carried out using groups of two rats (1 of each sex). The results obtained suggested that the LD50 would be approximately 2000mg/kg.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 100 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 1 787 - < 2 468
- Mortality:
- yes, see table below
- Clinical signs:
- other: Lethargy was observed starting at the lowest dose levels. In addition, lacrimation (at 1500 and 2000 mg/kg) and piloerection (at 2000 mg/kg) was observed in some animals.
- Gross pathology:
- No Abnormalities detected
Any other information on results incl. tables
MORTALITIES
| mortality | ||||
| Dose (mg/kg bw) | males | females | combined deaths | % |
| 500 | 0/5 | 0/5 | 0/10 | 0 |
| 1000 | 0/5 | 0/5 | 0/10 | 0 |
| 1500 | 0/5 | 1/5 | 1/10 | 10 |
| 2000 | 1/5 | 3/5 | 4/10 | 40 |
| 2500 | 4/5 | 5/5 | 9/10 | 90 |
| 3000 | 4/5 | 4/5 | 8/10 | 80 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
