Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Dibutyl carbonate
EC Number:
208-816-0
EC Name:
Dibutyl carbonate
Cas Number:
542-52-9
Molecular formula:
C9H18O3
IUPAC Name:
dibutyl carbonate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: yes, Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/l dry substance and aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/l dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min

Test conditions

Test temperature:
20 ± 2 °C
pH:
8.0
Dissolved oxygen:
Oxygen concentration during aeration: > 2,5 mg/l
Oxygen concentration immediately before measurement: > 6,5 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC20 (180min) = 2.2 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes