Trichloro-12,24-dihydro-5H-naphtho[2,3-h]naphth[2'',3'':6',7']indolo[2',3':6,7]anthra[2,1,9-mna]acridine-5,10,13,18,25-pentone

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

July 20, 2018 - July 27, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Key result

Water solubility:

< 1.08 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

63 g/L

Incubation duration:

24 h

Temp.:

30 °C

pH:

5.6

Key result

Water solubility:

< 1.08 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

63 g/L

Incubation duration:

48 h

Temp.:

30 °C

pH:

5.5

Key result

Water solubility:

< 1.08 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

61 g/L

Incubation duration:

72 h

Temp.:

30 °C

pH:

5.6

Preliminary   test

Three preliminary tests were carried out with specified amounts of the test item and 100 ml and 500 ml demineralized water, respectively. After each addition of an amount of water, the mixture was stirred for at least 24 hours, and visually checked for any undissolved particles.

Table3: Results of the preliminary test

Amount of test item / mg	Total volume of demineralized water / mL	Appearance of mixture
10.4	100	not dissolved
11.7	500	not dissolved
4.1	500	not dissolved

The preliminary test showed that the water solubility of the test item is < 10 mg/L.As the item is insoluble in organic solvents according to sponsor’s information, it is impossible to coat the sea sand of the columns with the test item. For this reason, the column elution method is not applicable and the water solubility had to be determined by the flask method.

 Flask method

The flask method was performed as described above. The data for the evaluation are given in Table 4. After the equilibration time precipitate was observed in the flasks of the experiments excluding in the blank experiment. The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given in Table 4.

 Table4: Water solubility experiments of the test item

Experiment	24 h experiment	48 h experiment	72 h experiment	Blank experiment (72 h)
Amount of test item / mg	6.3	6.3	6.1	--
Amount of demineralized water / mL	100	100	100	100
Stirring time at 30 °C / h	24	48	72	72
pH at ambient temperature	5.6	5.5	5.6	5.8
Dilution factor	1	1	1	1
Measured concentration / mg/L	< 1.08 / < 1.08	< 1.08 / < 1.08	< 1.08 / < 1.08	-- / --
Mean concentration / mg/L	< 1.08	< 1.08	< 1.08	--
Water solubility / mg/L	< 1.08	< 1.08	< 1.08	--

The water solubility of the test item at 20 °C was determined to be lower than the quantification limit (< 1.08 mg/L) of the analytical method.

Executive summary:

The water solubility c_S of the test item Vat Green 13 at a temperature of 20 °C was determined according to the flask method. It was found to be:

                                                                 c_S < 1.08 mg/L

Description of key information

The water solubility c_S of the test item Vat Green 13 at a temperature of 20 °C was determined according to the flask method. It was found to be:

                                                                 c_S < 1.08 mg/L

The above mentioned value is based on the TOC measured in water. However, not only the test item but also (more) soluble impurities are included in the TOC measurement. Therefore, the water solubility of the test item was calculated with WSKOW v1.42 included in EpiSuite 4.1.

The water solubility of Vat Green 13 was calculated to be 1.0 E-10 mg/L at 25°C.

Key value for chemical safety assessment

Water solubility:

0 mg/L

at the temperature of:

25 °C