Reaction Mass of Didodecyl nonylphenyl phosphite and Dodecyl bis(4-nonylphenyl) phosphite and 4-nonylphenyl ditridecyl phosphite and Bis(4-nonylphenyl) tridecyl

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 87/302/EEC page 89-94 Algal Inhibition test

Principles of method if other than guideline:

Determination of EbC 50 (0-72 h) : Concentration at which reduced the growth of algae by 50% in relation to control.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Didodecyl nonylphenyl phosphite

EC Number:

252-457-2

EC Name:

Didodecyl nonylphenyl phosphite

Cas Number:

35239-35-1

Molecular formula:

C39-H73-O3-P

IUPAC Name:

Phosphorous acid, didodecyl nonylphenyl ester

Constituent 2

Reference substance name:

Dodecyl bis(4-nonylphenyl) phosphite

EC Number:

284-117-4

EC Name:

Dodecyl bis(4-nonylphenyl) phosphite

Cas Number:

84787-76-8

Molecular formula:

C42-H71-O3-P

IUPAC Name:

Phosphorous acid, dodecyl bis(4-nonylphenyl) ester

Constituent 3

Reference substance name:

4-nonylphenyl ditridecyl phosphite

EC Number:

284-119-5

EC Name:

4-nonylphenyl ditridecyl phosphite

Cas Number:

84787-77-9

Molecular formula:

C41-H77-O3-P

IUPAC Name:

Phosphorous acid, 4-nonylphenyl ditridecyl ester

Constituent 4

Reference substance name:

Bis(4-nonylphenyl) tridecyl phosphite

EC Number:

284-120-0

EC Name:

Bis(4-nonylphenyl) tridecyl phosphite

Cas Number:

84787-78-0

Molecular formula:

C43-H73-O3-P

IUPAC Name:

Phosphorous acid, bis(4-nonylphenyl) tridecyl ester

impurity 1

Reference substance name:

Tris(nonylphenyl) phosphite

EC Number:

247-759-6

EC Name:

Tris(nonylphenyl) phosphite

Cas Number:

26523-78-4

IUPAC Name:

tris(2-nonylphenyl) phosphite

impurity 2

Reference substance name:

Didodecyl phosphonate

EC Number:

244-325-8

EC Name:

Didodecyl phosphonate

Cas Number:

21302-09-0

Molecular formula:

C24H51O3P

IUPAC Name:

Di(n-dodecyl)hydrogen phosphate

impurity 3

Reference substance name:

Phenol, 4-nonyl-, branched

EC Number:

284-325-5

EC Name:

Phenol, 4-nonyl-, branched

Cas Number:

84852-15-3

Molecular formula:

C15H24O

IUPAC Name:

4-Nonylphenol, branched

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Composite samples of each test concentration were drawn by mixing identical volumes of the test. Solutions taken from the approximate center of the test vessels.
They were taken inunediately before exposure and after 72 hours exposure and kept at - 18*'C to - 22°C until analysis.

Test solutions

Vehicle:

yes

Remarks:

28.8 mg N,N-dimethylformamide(DMF)/l and 1.2 mg polyoxy-ethylene-sorbitanmonooleate {TWEEN 80)/L

Details on test solutions:

0.123, 0.37, 1.1, 3.3, 10 and 30 mg/L (nominal)
<0.05, 0.2, 0.5, 1.7, 7.7 and 18.8 mg/L (actual)

Test organisms

Test organisms (species):

Scenedesmus sp.

Details on test organisms:

Collection of algae cultures
Pflanzenphysiologisches Institut
University
Nikolausberger Weg 18
D-3400 Göttingen
Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

23 ± 1°C.

pH:

7.8-7.9 initial
8.2-9.1 after 72 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

4.3 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

95 % confidence llmit: 0.49 - 23.2 mg/l

Reported statistics and error estimates:

The EC-50 values were calculated according to the maximun likelihood method, probit model (Mc Cullagh, P., Neider, J.A., 1983: Generalized linear modeis, Chapman & Hall, London)

Applicant's summary and conclusion

Conclusions:

EC50 was 4.3 mg/L after 72 hours.

Executive summary:

EC50 was 4.3 mg/L after 72 hours.