Hexadecyltrimethylammonium toluene-p-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Method similar to guideline but no analytical, and test species is not the standard species

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

no chemical analysis; Goldorfen is not the standard test species but is among those often tested

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): p-toluene sulfonic acid
- Substance type: organic
- Physical state: liquid
- Analytical purity: 65% active ingredient
- Purity test date: not indicated
- Lot/batch No.: not indicated
- Storage condition of test material: not indicated

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

diluted in water only

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfen
- Source: Paul Eggers, 2345 Hohenwestedt
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 5.5 - 6.6 cm
- Weight at study initiation (mean and range, SD): 1.5-2.7 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: no indicted
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min
- Feeding frequency: not indicated
- Health during acclimation (any mortality observed): not indicated

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

114 mg CaCO3/L

Test temperature:

20 +/- 1 C

pH:

7.5 - 8.2 (except at 500 mg/L where it was 5.7)

Dissolved oxygen:

8.5 - 9.2 mg/L

Salinity:

N/A

Nominal and measured concentrations:

Nominal - 0, 10, 100 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium with 20L solution
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 20L
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Ca/mg ratio: 0.7
- Na/K ratio: 21
- Intervals of water quality measurement: 0, 2, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality daily; observations regularly

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5-10X
- Range finding study: not indicated

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

>325 mg/L based on active ingredient

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

Test substance was not toxic at 500 mg/L (325 mg/L active substance), the highest concentration tested up to 96hours.

Description of key information

Test substance was not toxic at 500 mg/L (325 mg/L active substance), the highest concentration tested up to 96hours.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

500 mg/L