1,4-butanediyl diacrylate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

only 3 animals / sex used in highest dose

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-butanediyl diacrylate

EC Number:

213-979-6

EC Name:

1,4-butanediyl diacrylate

Cas Number:

1070-70-8

Molecular formula:

C10H14O4

IUPAC Name:

4-(prop-2-enoyloxy)butyl prop-2-enoate

Details on test material:

- Name of test material (as cited in study report): Butandioldiacrylat
- Analytical purity: > 90 %

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

rats: SPF-breed; supplyling company: WIGA, Sulzfeld
diet: Herlian MRH-concentrated feed; suppyling company: H. Eggersmann, Rinteln/Weser; ad libitum
water: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

olive oil

Details on dermal exposure:

TEST SITE
- Area of exposure: ~49 cm2
- Type of wrap if used: inert foil; fixed with adhesive tape

REMOVAL OF TEST SUBSTANCE
- Washing: warm water or water/lutrol mixture; drying using tissue paper
- Time after start of exposure: 24 h

TEST MATERIAL
- Concentration: 50% for concentration 400 mg/kg(in olive oil); for all other concentrations the undeluted test substance was applied.

Duration of exposure:

24 h

Doses:

400, 800, 1250, 2000 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female (exception was dose group 2000 mg/kg bw: 3 male and 3 female)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Weighing: one time at the beginning
- Necropsy of survivors performed: yes
- Fur was removed by shaving 15-24 hours before the beginning of the test. Only animals with healthy und undamaged skin were used for the test .
- Other examinations performed: clinical signs, necropsy

Statistics:

no data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 400 - < 800 mg/kg bw

Mortality:

see table below

Clinical signs:

other: Resorptive Symptoms of poisoning: unsteady breathing; apathy; staggering; ruffled fur local irritation: 24 h following application significant primary irritations can be detected, which turns into necrosis on day 4.

Gross pathology:

animals that died:
heart: acute dilatation (right side); acute congestive hyperemia;
lung: in some cases acute inflation of medium grade
surviving animals:
no abnormality detected

Any other information on results incl. tables

Mortality

Dose (mg/kg bw)	conc. (%)	# animals	died within 1 h	died within 24 h	died within 48 h	died within 7 d	died within 14 d
2000	100	3 male	0/3	0/3	0/3	2/3	2/3
		3 female	0/3	3/3	3/3	3/3	3/3
1250	100	5 male	0/5	5/5	5/5	5/5	5/5
		5 female	0/5	5/5	5/5	5/5	5/5
800	100	5 male	0/5	5/5	5/5	5/5	5/5
		5 female	0/5	5/5	5/5	5/5	5/5
400	50	5 male	0/5	0/5	0/5	0/5	0/5
		5 female	0/5	1/5	1/5	1/5	1/5

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria