Registration Dossier
Registration Dossier
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EC number: 213-584-9 | CAS number: 989-38-8
Endpoint summary
Administrative data
Description of key information
The read-across substance, 3,6-bis(ethylamino)-9-[2-(methoxycarbonyl)phenyl]-2,7-dimethylxanthylium chloride, was not readily biodegradable under the conditions of the modified Sturm test.
This result is supported with the result of a QSAR calculation using EpiSuite v.4.11 for the target substance (CAS 989-38-8).
Additional information
A ready biodegradability study with 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium chloride (CAS 989-38-8) is not available. Consequently, read-across was applied using a characteristically similar compound: 3,6-bis(ethylamino)-9-[2-(methoxycarbonyl)phenyl]-2,7-dimethylxanthylium chloride (CAS 3068-39-1).
The ready biodegradability of the source substance was assessed in a modified Sturm test conducted in accordance with the standardised guidelines OECD 301B, EU Method C.4-C, ISO 9439 and ISO 10634 under GLP conditions.
The test material was tested in duplicate at a target concentration of 17 mg/L, corresponding to 12 mg TOC/L. The organic carbon content was based on the molecular formula. The theoretical CO2 production (ThCO2) of the test material was calculated to be 2.56 mg CO2/mg.
The study consisted of six bottles: 2 inoculum blanks (no test material), 2 test bottles (test material), 1 positive control (sodium acetate) and 1 toxicity control (test material plus sodium acetate).
Since the test material was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to the 2-litre test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test material. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test to ensure optimal contact between the test material and test medium. Test duration was 28 days (last CO2-measurement on day 29).
The relative biodegradation values calculated from the measurements performed during the test period revealed 5 and 2 % biodegradation of the test material (based on ThCO2) for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60 % biodegradation within a 10-day window) was not met. In the toxicity control, the test material was found to not inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
The test material was not readily biodegradable under the conditions of the modified Sturm test.
Additionally, the ready biodegradability of the target substance was calculated using BIOWIN v4.10 as part of EPISuite v4.11 from US Environmental Protection Agency. Based on the results of BIOWIN v.4.11 the test substance is predicted to be not readily biodegradable.
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