Condensation products of fatty acids, tall oil with 2-amino-2-ethylpropanediol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

The reported study is conducted with the analogue substance Alkaterge-T, which s made by condensing trometamol and Tall Oil Fatty Acids. Altalaterge E is made by condensing AEPD and Tall Oil Fatty Acids

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

no

Specific details on test material used for the study:

C-14 labelled test material was used in the study

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The substance solution was inoculated with a few drops of inoculum from the discharge of a community water treatment plant. This completed test solution was distributed into the test vessels in exactly determined volumes so that duplicate samples were available for the determination of the O2 content. In addition, a corresponding number of test vessels were set up with nutrient solution, with nutrient solution + inoculum and a comparison substance.

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Details on study design:

The chemical to be investigated was dissolved in a concentration of approximately 2 mg/L in a mineral nutrient medium. The latter was prepared from aerated deionized water (9 mg of 02/L) and from the corresponding nutrient salt and trace element stock solutions. The substance solution was inoculated with a few drops of inoculum from the discharge of a community water treatment plant. This completed test solution was distributed into the test vessels in exactly determined volumes so that duplicate samples were available for the determination of the O2 content. In addition, a corresponding number of test vessels were set up with nutrient solution, with nutrient solution + inoculum and a comparison substance. The test bottles were stored in the dark at 20-21°C and the oxygen content was determined on day 0 (beginning), day 5, day 15, day 28 (end) using an oxygen electrode. From the particular O2 content of the nutrient solution, the nutrient solution with inoculum and the test substance solution, the oxygen demand was determined for the test substance. From this, one was able to calculate the theoretical oxygen demand of biodegradation in %.

Dt = mg of BODx / L * 100 dvided by mg of substance / L * TOB

Dt = biodegradation in % of the test substance
BODx = biological oxygen consumption after x days
TOB = theoretical oxygen consumption

Reference substance:

acetic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

32

Sampling time:

28 d

Remarks on result:

other: not readily biodegradable

Key result

Parameter:

BOD5

Value:

0.9 mg O2/g test mat.

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

The test substance is not readily biodegradable in the closed bottle test.

Executive summary:

The test substance is not readily biodegradable in the closed bottle test.

Description of key information

Alkaterge E can be considered not readily biodegradable, based on the results of a biodegradation test (closed bottle) with the analogue substance Alkaterge T.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater