Tricopper bis(orthophosphate)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2004-11-23 to 2004-12-03

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2011

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

yes

Remarks:

The water accommodated fraction (WAF) was not prepared for each treatment concentration by weighing the respective load, but by preparation of a WAF of the highest tested concentration (100 mg/L). By using this solution the treatments were prepared.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dicopper hydroxide phosphate

EC Number:

235-285-2

EC Name:

Dicopper hydroxide phosphate

Cas Number:

12158-74-6

Molecular formula:

Cu2HO5P

IUPAC Name:

copper(2+) hydroxide phosphate (2:1:1)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was stored in a closed vessel at room temperature. Due to the poor water solubility, the water-accommodated fractions of the concentrations to be tested were prepared. This was done by shaking the nominal load with the appropriate amount of dilution water for 24 hours, followed by filtration. The resulting solution was used for treatments to be tested.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation: 0-24 h
- Food type: green alga (Desmodesmus subspicatus)
- Conditions: Kept in M4-Medium following ELENDT, five adults per liter and transferred into fresh medium twice a week

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Observation at 24 and 48 hours

Test conditions

Test temperature:

20 ± 2 °C

pH:

7.9 - 8.2

Dissolved oxygen:

8.3 - 8.7 mg/L

Nominal and measured concentrations:

Main study (nominal): 100, 46, 22, 10, 4.6, 2.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50-mL-beakers (DIN, HF)
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic dilution water following ISO 6341

OTHER TEST CONDITIONS
- Photoperiod: lighting cycle 16h/8h (light/dark)

EFFECT PARAMETERS MEASURED: 24h and 48h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.17

- Range finding study
- Test concentrations (mg/L): 100, 10, 1, 0.1, 0.01, 0.001

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

22.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

None

Results with reference substance (positive control):

Validity
The 24h-EC50i of the reference K2Cr2O7 should lie between 0.6 and 2.4 mg/L. A 24h-EC50i of 1.2 mg/L was determined in a study performed recently.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24 and 48 hour NOEC of dicopper hydroxide phosphate was determined to be 4.6 mg/L (nominal).