Reaction mass of 29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32 copper and hydrogen [29H,31H-phthalocyaninesulphonato(3-)-N29,N30,N31,N32]cuprate(1-), compound with dodecylamine (1:1)

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 June 2016 - 06 July 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

Storage: Controlled room temperature (15-25 °C, below 70 RH%)

Radiolabelling:

not specified

Test temperature:

25 °C

Details on study design: HPLC method:

- Type, material and dimension of analytical (guard) column: Lichrospher 100 CN, 250×4.6 mm, 5μm
- Detection system: 220 nm (standard), 340 nm (test item)

MOBILE PHASES
- Type: Acetonitrile: ultrapure water = 1:1

REFERENCE SUBSTANCES
- Identity: Sodium nitrate, Acetanilide, Methyl benzoate, Linuron, Naphthalene, Fenthion, α-Endosulfan, 4,4’-DDT

Key result

Type:

log Koc

Value:

< 1.25 dimensionless

Temp.:

25 °C

Matrix:

soil or sewage sludge matrices

Details on results (HPLC method):

- Retention times of reference substances used for calibration: Sodium nitrate 1.49 min, Acetanilide 4.08 min, Methyl benzoate 5.02 min, Linuron 5.9 min, Naphthalene 6.55 min, Fenthion 7.85min, α-Endosulfan 9.21 min, 4,4’-DDT 12.98 min,
- Details of fitted regression line: log k' vs. log Koc)
- Average retention data for test substance: B331 0.97 min

Validity criteria fulfilled:

not specified

Conclusions:

The test item eluted before the deadtime standard (sodium nitrate), therefore an accurate value of log Koc could not be given.
The calculated log Koc: <1.25

Description of key information

An accurate determination of the Koc / log Koc could not be obtained. The log Koc is estimated to be < 1.25

Additional information

An adsorption coefficient determination was conducted using the HPLC method (CiToxLAB, 2017, Study 15/361 -386AN) to calculate the soil adsorption coefficient for B331. THe determination was conducted according to EC method C19 and OECD test guideline 121, and in compliance with GLP.

In the HPLC determination the test item peak eluted before the deadtime standard therefore an accurate value could not be calculated. It is estimated that the logKoc <1.25.