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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Version / remarks:
May 1996 (Classement indice AFNOR: T 90 301)
GLP compliance:
no
Remarks:
performed before 2008

Test material

Constituent 1
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Name in the report: X113
- Source and lot/batch No.of test material:D581105*
- pH : 7.47
- acid concentration: 0.0971 mol/l
- base concentration: 0.0983 mol/l
- Expiration date of the lot/batch: 13 November 2000

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Stability under test conditions: stable in water, instable after repeated contact to air
- Solubility and stability of the test substance in the solvent/vehicle: 100% - stable in water
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: no

FORM AS APPLIED IN THE TEST (if different from that of starting material): liquid form (diluted at 6% into a saline solution)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Remarks:
water

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
20-22°C
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
5 654 mg/L
Nominal / measured:
estimated
Conc. based on:
act. ingr. (total fraction)
Basis for effect:
mobility
Results with reference substance (positive control):

- Other: not documented
Reported statistics and error estimates:
Confiance level at 95% is between 4404 mg/l and 7618 mg/l for EC50 (24h) (from method of moving mean recommended into the followed guideline)

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
X330 was tested according to EN ISO 6341(1996) (NFT 90301) in order to determine which concentration of the active ingredient stops 50% of Daphnia Magna mobility after 24 hours of exposure. The estimated EC50 (24 hr) was determined at 5654 mg/l with a confidence interval at 95% between 4404 and 7618 mg/l.
Executive summary:

X330 was tested according to EN ISO 6341(1996) (NFT 90301) in order to determine which concentration of the active ingredient stops 50% of Daphnia Magna mobility after 24 hours of exposure. The estimated EC50 (24 hr) was determined at 5654 mg/l with a confidence interval at 95% between 4404 and 7618 mg/l.