Reaction products of diazotised Benzenesulfonic acid, 2-amino-5-[(2-hydroxyethyl)sulfonyl]- , subsequently coupled with 3,5-Diaminobenzoic acid, further coupled with diazotised Ethanol, 2-[(4-aminophenyl)sulfonyl]-, 1-(hydrogen sulfate), sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From March 16, 2016 to October 25, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: National Chiayi University Freshwater Biological Resource Center

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Nominal and measured concentrations:

For the limit test: 100.00 mg/L
For the definitive test: 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L

Details on test conditions:

- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for limit and definitive test
- No. of vessels per control (replicates): 4 for limit and definitive test
- Photoperiod: 16-hr light: 8-hr dark

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

In range-finding test, the 0% of control organisms were immobilized and the dissolved oxygen concentration at the end of the test was 7.99-8.04 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. The results of the range-finding test showed that EC₅₀ was greater than 100.00 mg/L.

In limit and definitive test, the results showed below:

 

Table 1. The numbers immobilized for the limit test on day 1 and 2

Days	Group (mg/L)		Numbers of no immobilized	Numbers of activity immobilized	Numbers of discoloration	Numbers of trapping at surface of water
Day 1	Control group	0.00	19	1	0	0
	Test group	100.00	17	3	0	0
Day 2	Control group	0.00	19	1	0	0
	Test group	100.00	15	5	0	0

 

Table 2. The percentages immobilized and EC₅₀ for the limit test

Group (mg/L)		Percentages immobilized (%)			EC50 (mg/L)
		Day 0	Day 1	Day 2
Control group	0.00	0.00	5.00	5.00	－
Test group	100.00	0.00	15.00	25.00	>100.00

 

Table 3. Temperature, dissolved oxygen and pH value for the limit test

Group (mg/L)		Day 0			Day 2
		Temperature (℃)	Dissolved oxygen (mg/L)	pH value	Temperature (℃)	Dissolved oxygen (mg/L)	pH value
Control group	0.00	20.0	8.25 ± 0.21	7.39 ± 0.02	20.0	8.35 ± 0.05	7.42 ± 0.03
Test group	100.00		8.55 ± 0.04	7.52 ± 0.03		8.26 ± 0.09	7.39 ± 0.05

 

Table 4. The numbers immobilized for the definitive test on day 1 and 2

Days	Group (mg/L)		Numbers of no immobilized	Numbers of activity immobilized	Numbers of discoloration	Numbers of trapping at surface of water
Day 1	Control group	0.00	19	1	0	0
	Test group	100.00	20	0	0	0
		50.00	20	0	0	0
		25.00	20	0	0	0
		12.50	20	0	0	0
		6.25	20	0	0	0
Day 2	Control group	0.00	19	1	0	0
	Test group	100.00	20	0	0	0
		50.00	20	0	0	0
		25.00	19	1	0	0
		12.50	20	0	0	0
		6.25	20	0	0	0

 

Table 5. The percentages immobilized and EC₅₀ for the definitive test

Group (mg/L)		Percentages immobilized (%)			EC50 (mg/L)
		Day 0	Day 1	Day 2
Control group	0.00	0.00	5.00	5.00	－
Test group	100.00	0.00	0.00	0.00	>100.00
	50.00	0.00	0.00	0.00
	25.00	0.00	0.00	5.00
	12.50	0.00	0.00	0.00
	6.25	0.00	0.00	0.00

 

Table 6. Temperature, dissolved oxygen and pH value for the definitive test

Group (mg/L)		Day 0			Day 2
		Temperature (℃)	Dissolved oxygen (mg/L)	pH value	Temperature (℃)	Dissolved oxygen (mg/L)	pH value
Control group	0.00	20.0	8.42 ± 0.15	7.44 ± 0.04	20.0	8.31 ± 0.17	7.05 ± 0.07
Test group	100.00		8.46 ± 0.04	7.21 ± 0.05		8.27 ± 0.05	7.29 ± 0.04

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, the 48-hr EC50 of CR SB87 was >100 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100123001EN which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CR SB87 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of CR SB87 was >100 mg/L.

Description of key information

The 48-hr EC50 of CR SB87 was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In range-finding, limit test and definitive test, not more than 10 % of the Daphnia were immobilized in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O₂/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Under the conditions of this test, the 48-hr EC50 of CR SB87 was >100 mg/L.