Rape oil, polymer with tung oil

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

not applicable

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Rape oil, polymer with tung oil

EC Number:

606-051-0

Cas Number:

185323-46-0

IUPAC Name:

Rape oil, polymer with tung oil

Specific details on test material used for the study:

PhytoVie Defense, INCI: Brasslca Campestris/Aleurites Fordi Oil
Copolymer, Lot# 520818

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

natural water: freshwater

Details on inoculum:

inoculum: Surface water from Skokie, IL water district.
- proportion and nature of industrial waste water in sewage: unknown, discharge from waste treatment facility within 1 mile.
- test duration and temperature: 28 days or as indicated, 22C +/- 2C
- bacterial inoculum -1E5 CFU/ml

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

20 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

32

Sampling time:

28 d

Details on results:

Sodium acetate control achieved 76% degradation after 28 days.

Any other information on results incl. tables

The OECD 301B method is designed to provide the screening of chemicals for ready biodegradability in an aerobic aqueous medium. Samples are required to achieve a threshold of 60% degradation based on the maximum available carbon from a given sample formulation. Total carbon is determined analytically for each sample and used as the reference for the determination of the percentage of carbon dioxide (% ThCO₂) produced by microbiological degradation.

 

For the purpose of determining biological degradation, two criteria can be achieved. Ready Biodegradability can be achieved by obtaining the 60% threshold within a 10 day-window within the 28 days of testing. The second criteria, Ultimate Biodegradation, can be achieved if the amount of biodegradation meets or exceeds the 60% threshold at a time point determined in the test (e.g. when the rate of degradation reaches a plateau). For each of these criteria, when achieved, the sample also achieves the requirements needed for classification as 'Inherent Biodegradability'.

 

One test sample was submitted for OECD 301B biodegradation testing. The result data and graphs were analyzed by curve fit to establish a plateau for the rate of biodegradation.

 

Sample 1 - PhytoVie Defense, INCI: Brassica Campestris/Aleurites Fordi Oil Copolymer, Lot# 520818 - was a straw colored clear hydrophobic liquid and was introduced into the test system dispersed onto silica gel. The sample graph attached shows the test chamber carbon dioxide (C02) measurement as the percent of theoretical maximum (% ThC02). Average values are plotted with the standard deviation (+/- SD) for the time course of the test. Curve fit is applied to calculate the predicted fit (blue line). If present, (green) shading below the curve fit applies to the biodegradation requirement (10 to 60% ThC02) for the determination of Biodegradability and shows that the required degradation amount has been met.

Ready Biodegradability requires that this degradation occur within a 10-day window during the test

duration of 28 days.

 

The test sample did not achieve the requirements for Ready Biodegradability by the OECD 301B standard. The test sample achieved an inherent degradation of 32% ThCO₂within 28 days of the test timeframe. A degradation plateau was not achieved within the 28 days of testing.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The test sample achieved an inherent degradation of 32% ThCO2 within 28 days of the test timeframe.