5-amino-2-chloro-N-(2,4-dimethylphenyl)benzenesulphonamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991: May 14 to October 8

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(1984)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

no

Analytical monitoring:

not specified

Details on sampling:

Not specified

Vehicle:

yes

Details on test solutions:

Not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout (Salmo gairdneri)
- Source: P. Hohler Forellenzucht, CH-4314 Zeiningen/Switzerland
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 35-55 mm
- Weight at study initiation (mean and range, SD): 1.1-1.5g
- Method of breeding:
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food during acclimation:
- Feeding frequency during acclimation:
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

FEEDING DURING TEST
- Food type:
- Amount:
- Frequency:

Test type:

not specified

Water media type:

other: dionized water in which the following amounts of salt have been added: 222.0 mg/L of CaCl2; 60.1 mg/L of MgSO4; 64.8 mg/L of NaHCO3; 5.75 mg/L of KCl

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not specified

Hardness:

14 dH°

Test temperature:

15.0°C

pH:

7.8-8.1

Dissolved oxygen:

10.2-12.2 mg/L

Salinity:

The test medium is made of dionized water in which the following amounts of salt have been added: 222.0 mg/L of CaCl2; 60.1 mg/L of MgSO4; 64.8 mg/L of NaHCO3; 5.75 mg/L of KCl

Conductivity:

Not specified

Nominal and measured concentrations:

Nominal: 0; 0.1; 1 and 10 mg/L

Details on test conditions:

TEST SYSTEM
- Aeration: The water was continuously aerated uslng compressed alr.
- No. of organisms per vessel: 10 fishes were exposed to the test article on all-glass containers containing 15L test medium
- Biomass loading rate: the fish biomass was 0.8-1.0 g/L test medium.

TEST MEDIUM / WATER PARAMETERS
A stock suspenslon was prepared by maktng up 1 g test article to 10 mL with acetone. Quantitles of 1.5, 0.15 and 0.015 mL of thls stock suspension were added to 15L with test medium, adding the required quantltles of acetone to a concentratlon of 0.01% by volume. In addition to the treated fish, control flsh were exposed to untreated test medium containing 0.01% by volume of acetone and to the test medium alone.

OTHER TEST CONDITIONS
The experiment was conducted under Standard Laboratory Condltions ln an alr condltioned
room in and light/dark cycle of 12 hours of artlflclal fluorescent light (about 500-1500 Lux) per day.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 - <= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 1 - <= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No clinical signs were observed up to 1.0 mg/L.
The mortality was as follows:
0/10 in control group with and witout acetone and in test item group at 0.1 and 1.0 mg/L
10/10 in test item group at 10.0 mg/L

Validity criteria fulfilled:

yes

Remarks:

mortality of the fish in the control is <10%

Conclusions:

ACDMA has been tested for its acute toxicity to Rainbow trout on test performed according to OECD TG 203 with determination of NOEC (96h) of 1 mg/L; LOEC (96h) of 1-10 mg/L and LC50 (96h) between 1 and 10 mg/L

Executive summary:

ACDMA has been tested for its acute toxicity to Rainbow trout on test performed according to OECD TG 203 with determination of NOEC (96h) of 1 mg/L; LOEC (96h) of 1-10 mg/L and LC50 (96h) between 1 and 10 mg/L

Description of key information

ACDMA has been tested for its acute toxicity to Rainbow trout on test performed according to OECD TG 203 with determination of NOEC (96h) of 1 mg/L; LOEC (96h) of 1-10 mg/L and LC50 (96h) between 1 and 10 mg/L

Additional information

With a 96h LC50 > 1 and ≤10 mg/L for fish, the test item is classified as Aquatic chronic 2 (H411).