Proteinase, Aspergillus acid

Density

Administrative data

Endpoint:

density, other

Remarks:

density

Type of information:

other: Category approach

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Density data have been generated for various protein famili es. The data published in the cited book comes from 20 different protein families and they all have densities in the range from 1.32 g/mL to 1.42 g/mL. The density of the aspergillopepsin I has not been det ermined but since it is a protein it is expected, with very high reliability that it has density in this range.

Data source

Reference

Reference Type:

review article or handbook

Title:

Proteins: structures and molecular properties

Author:

Thomas E. Creighton

Year:

1993

Bibliographic source:

W.H.Freeman and Company; ISBN 0-7167-2317-4

Materials and methods

Principles of method if other than guideline:

Determination of the sedimentation constant allows calculation of the density using the Svedberg equation. This has been performed in peer reviewed articles and summarised in Creighton "Proteins: structure and molecular properties" (1993).

GLP compliance:

not specified

Type of method:

other: See "Principles of method if other than guideline"

Test material

Test material information

Constituent 1

Reference substance name:

Protein as a constituent of enzyme deriving from the fermentation or extraction process

Molecular formula:

Not available. See remarks.

IUPAC Name:

Protein as a constituent of enzyme deriving from the fermentation or extraction process

Details on test material:

20 different proteins from different families have been analysed in peer reviewed articles and summarised in Creighton "Proteins: structure and molecular properties" (1993) .

Results and discussion

Density

Key result

Type:

density

Density:

> 1.33 - < 1.42 g/cm³

Temp.:

20 °C

Remarks on result:

other: density range for 20 different proteins from different protein-families

Any other information on results incl. tables

Creighton reviews density data retrieved from structurally and fuctionally different proteins. They all have densities in the range between1.32 and 1.42 g/ml.

We ascertain that Aspergillopepsin I also has a density in this range since it is a protein, even though not experimentally determined.

Applicant's summary and conclusion

Conclusions:

Aspergillopepsin I is a protein and therefore it can be assumed that this has density within the range 1.32 - 1.42 g/ml.

Executive summary:

Densities have been determined for a wide range of different proteins. This analysis showed that the density range is between 1.32 and 1.42 g/ml. Since Aspergillopepsin I is a protein, we conclude that this protein also has density within this range.