Indigofera tinctoria, ext.

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10.4.2018-14.4.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2012

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: OECD guidance no 23 on aquatic toxicity testing of difficult substances.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Indigofera tinctoria, ext.

EC Number:

283-892-6

EC Name:

Indigofera tinctoria, ext.

Cas Number:

84775-63-3

Molecular formula:

n/a

IUPAC Name:

Indigofera tinctoria, ext.

Test material form:

solid: particulate/powder

Details on test material:

Batch number: OIG/17//I/1

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Details on test solutions:

Fresh extraction of the test concentration was prepared at room temperature in normal lighting. The test concentration was stirredwas stirred at a speed that created a small vortex on the surface for 24hours in a covered glass beaker. After being allowed to settle for 1 hour, the volume was filtered in two steps.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia magna culture is a Clone-A originating from the Norwegian Institute of Water Research (NIVA) Norway.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20±2°C

pH:

7,7-8,1

Dissolved oxygen:

5,0-8,6

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 - <= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The immobilisation of the Daphnia Magna at the concentration 100 mg/l was 0% after 48hours exposure, and therefore the NOEC-value is determined to be 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes