Lawsonia inermis, ext.

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10.4.2018-14.4.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2012

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: OECD series on testing and assessment No 23 Guidance document on aquatic toxicity testing of difficult substances and mixtures.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Lawsonia inermis, ext.

EC Number:

284-854-1

EC Name:

Lawsonia inermis, ext.

Cas Number:

84988-66-9

Molecular formula:

na

IUPAC Name:

Lawsonia inermis, ext.

Test material form:

solid: particulate/powder

Details on test material:

Batch number:ONH/17/I/01

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Details on test solutions:

Fresh extraction of the test concentration was prepared at room temperature in normal lighting. Teh test concentration was stirred at a speed that created a small vortex on the surface for 24 hours in a covered glass beaker. After being allowed to settle for 1 hour, the volume was filtered in two steps.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia magna culture is a Clone-A originating from the Norwegian Institute of Water Research (NIVA), Norway. The Clone-A has been indentified by genotyping.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20±1°C

pH:

7,7-8,0

Dissolved oxygen:

5,7-8,6 mg/l

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 - <= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The immobilisation of the Daphnia magna at the concentration 100 mg/l was 0% after 48 hours and therefor the NOEC-valu is determinded to be 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes