Tetrasodium tripotassium (.my.-((2,2'-((3-imino-6-sulfonatophenyl-(N-methyl)-imino)bis((6-chloro-1,3,5-triazin-4,2-diyl)imino(2-hydroxy-5-sulfonato-3,1-phenylen)azo(phenylmethylen)azo))bis(4-sulfonatobenzoato))-dicuprate(7-)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June 08, 2000 to July 06, 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: ISO 9408:1991

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Municipal activated sludge from the waste water treatment plant of Mannheim/Baden-Wurttemberg (Germany), stored in laboratory wastewater treatment plant which was fed with municipal sewage. The sludge was washed and aerated for 24 hours and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance.
Test concentration in medium 1, 2 and 3: 100 mg/L
Volume of reaction mixture: 0.25 L
ThOD concentration in medium: 46.2 mg O2/L
ThOD per mg of test substance: 0.462 mg O2/mg test substance

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Initial conc.:

46.2 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

The monometric respiratory test is a static method for testing the ultimate aerobic biodegradability of a test substance in water. Mixtures of the test substance, a defined inorganic medium and an inoculum, which is not pre-adopted activated sludge of a municipal or laboratory waste water treatment plant, are incubated and aerated in a respirometer (Sapromat) at 22 ±1 °C up to 28 days. The oxygen used for the biodegradation of the test substance (biochemical oxygen demand, BOD) in continuously produced and measured by the test apparatus. Primary biodegradation is also calculated from supplemental specific chemical analysis made at the beginning and the end of incubation, and in addition ultimate biodegradation by DOC analysis. Any intermediates are identified, if possible.

Reference substance:

aniline

Key result

Parameter:

% degradation (DOC removal)

Value:

> 0 - < 10

Sampling time:

28 d

Key result

Parameter:

other: BOD of ThOD

Value:

> 0 - < 10

Sampling time:

28 d

Details on results:

Degree of biodegradation (BOD of ThOD) after 28 days: 0 – 10 %
Degree of substance specific elimination by UV-VIS after 28 days: 0 – 10 %
Degree of DOC elimination after 28 days: 0 – 10 %
The test substance is in this test poorly biodegradable.

Results with reference substance:

ThOD concentration of reference substance: 2.393 mg O2/mg reference substance

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Under the study conditions, the test substance is not considered to be readily biodegradable.

Executive summary:

A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301 F (manometric respirometry), EU Method C.4 and ISO Method 9408:1991, in compliance with GLP. The test substance, at 100 mg/L, was inoculated for 28 d in a pre-aerated microbial system collected from a municipal sewage plant. The sludge was washed and aerated for 24 h, then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance. Three assays were conducted. Under the study conditions, the test substance did not biodegrade during the test period. Measured BOD values (except for assay 3) corresponded well with the values found with DOC and specific chemical analysis. Assay 3 was not validated due to technical deficiencies (leaky) (Beimborn, 2000).

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

From August 17, 1993 to September 14, 1993

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Substance purity not specified

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Official Journal of the European Communities No L383 A, July 31, 1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC Directive 92/69/EEC

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Effluent of a municipal waste water treatment plant.

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation:

DOC removal

Preliminary study:

TOC: 212 mg/g
DOC: 214 mg/g

Key result

Parameter:

other: DOC removal (mg/L)

Value:

< 20

Sampling time:

28 d

Results with reference substance:

Stock solution: 431 mg/L
DOC value stock solution: 258 mg/L
> 90

Data evaluation:

If DOC decrease due to abiotic processes like stripping or adsorption may be excluded, the test substance is biodegradable. The DOC concentration at the end of the test is compared with the initial concentration. The degradation is calculated as per cent value, stated in 10%-steps and interpreted as follows:

DOC-decrease >70 % in 10 days (ready biodegradable)

DOC-decrease >70 % biodegradable

DOC-decrease 20-70 % moderately or partially biodegradable

DOC decrease <20 % poory biodegradable

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Under the study conditions, the test substance was not considered to be readily biodegradable.

Executive summary:

A study was conducted to evaluate the ready biodegradability of the test substance according to a modified OECD Guideline 301 E, EEC Directive 92/69/EEC and the Official Journal of the European Communities No L383 A, July 31, 1992, in compliance with GLP. The test substance at a concentration of 20 mg dissolved organic carbon (DOC)/L, a defined dilution water and, as inoculum, effluent of a laboratory waste water treatment plant fed with municipal sewage were mixed together and aerated up to 28 d at 20-25°C. Samples were taken at regular intervals to measure the DOC concentration according to DIN 38409 Part 3 (1983). Under the study conditions, the degree of biodegradation was lower than 20 mg DOC/L after 28 d. Under the study conditions, the test substance was therefore not considered to be readily biodegradable (Taeger, 1993).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

A study was conducted to evaluate the ready biodegradability of the test substance according to a modified OECD Guideline 301 E, EEC Directive 92/69/EEC and the Official Journal of the European Communities No L383 A, July 31, 1992, in compliance with GLP. The test substance at a concentration of 20 mg dissolved organic carbon (DOC)/L, a defined dilution water and, as inoculum, effluent of a laboratory waste water treatment plant fed with municipal sewage were mixed together and aerated up to 28 d at 20-25°C. Samples were taken at regular intervals to measure the DOC concentration according to DIN 38409 Part 3 (1983). Under the study conditions, the degree of biodegradation was lower than 20 mg DOC/L after 28 d. Under the study conditions, the test substance was therefore not considered to be readily biodegradable (Taeger, 1993).

A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301 F (manometric respirometry), EU Method C.4 and ISO Method 9408:1991, in compliance with GLP. The test substance, at 100 mg/L, was inoculated for 28 d in a pre-aerated microbial system collected from a municipal sewage plant. The sludge was washed and aerated for 24 h, then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance. Three assays were conducted. Under the study conditions, the test substance did not biodegrade during the test period. Measured BOD values (except for assay 3) corresponded well with the values found with DOC and specific chemical analysis. Assay 3 was not validated due to technical deficiencies (leaky) (Beimborn, 2000).