2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

EC Number:

248-514-6

EC Name:

2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

Cas Number:

27538-10-9

Molecular formula:

C7H10O3

IUPAC Name:

2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

22.1 to 22.2oC

pH:

8.0

Nominal and measured concentrations:

A range-finding test was carried out using the following concentrations of test substance: control; 0.1; 1; 10 and 100 mg.L-1 of test substance, with two replicates per concentration.
Based on the results obtained in the range-finding test, the definitive test was carried out with the following concentrations of test substance: control; 1; 3.2; 10; 32 and 100 mg.L-1.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

other: EyC50

Effect conc.:

7.27 mg/L

Conc. based on:

test mat.

Basis for effect:

other: yield

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

43.37 mg/L

Basis for effect:

growth rate

Results with reference substance (positive control):

The 72-hours EyC50 value of the reference substance potassium dichromate was 0.65 mg.L-1 (confidence limits at 95% = 0.59 to 0.71 mg.L-1), demonstrating that the toxic sensitivity of the algae was within the accepted range (ISO, 1989), which report toxicity ranged between 0.60 and 1.03 mg.L-1.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The objective of this study was to evaluate the toxicity of Furanone Homo on alga Pseudokirchneriella subcapitata. A range-finding test was conducted to find the concentrations that cause algae growth inhibition. Based on the results of the range-finding test, a definitive test was carried out with the following concentrations of test substance: 0 (control); 1; 3.2; 10; 32 and 100 mg.L-1. The test was incubated during 72 hours inside chambers with controlled conditions of luminosity, temperature, and agitation. The 72-hours EyC50 value was 7.27 mg.L-1 and the 72-hours ErC50 value was 43.37 mg.L-1. The highest test substance concentration which no observed significant adverse effect (NOEC) on algal growth was 1 mg.L-1 and the lowest test substance concentration at which was observed significant reducing effect on growth (LOEC) was 3.2 mg.L-1.