2-Fluor-4-[trans-4-(trans-4-propylcyclohexyl)-cyclohexyl]-phenylboronic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jun 11 – Sep 27, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The samples were taken from the biological phase of the study. Collecting, storage and handing over of the samples were the Study Director’s responsibility. The information concerning the samples was provided by the Study Director.
Duplicate samples from the freshly prepared test medium of the only test concentration and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the only test concentration and the control were collected at the end of the test (after 48 hours).
All samples were diluted by a factor of 2 with acetonitrile.
Additional samples of the control and the dilution solvent were taken at each sampling test start and test end without any sample treatment.

Vehicle:

no

Details on test solutions:

The test item was not well soluble in test water. To avoid physical effects of undissolved test item on the daphnids, no concentration above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 104.7 mg of test item in 1047 mL test water. The stock solution was shaken overhead for 24 hours in the dark to dissolve as much test item as possible. Then, the undissolved test item was separated by filtration (0.45 μm cellulose acetate filter) and the filtrate was used as test medium.
The test media was prepared just before introduction of the daphnids (= start of the test).

Test organisms (species):

Daphnia magna

Details on test organisms:

Strain/Clone: Daphnia magna, clone 5;
Age: 1.5 to 19.25 hours old
Source: The daphnids introduced in the test were taken from in-house laboratory culture.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 mg/L

Test temperature:

20.4 to 20.9 °C

pH:

7.9 to 8.0

Dissolved oxygen:

≥ 8.6 mg O2/L in in all treatment groups

Nominal and measured concentrations:

100 mg/L (nominal)
< LOQ (measured)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 48 hours of exposure no immobilisation of the test animals was observed in the control and the filtrate of the only tested test item concentration of nominal 100 mg test item/L.

Results with reference substance (positive control):

Results of the most recent Test with the Reference Item Potassium dichromate performed in January 2018
EC50 [mg/L]: 1.06 (24h) and 1.02(48 h)

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item to Daphnia magna was assessed in a static limit test. The 48 -hour NOEC was determined to be at least the at the concentration of the filtrate of nominal 100 mg test item/L. The 48-hour EC50 was clearly higher than the filtrate of nominal 100 mg test item/L.

Description of key information

OECD 202: EC50 > 100 mg/L (nominal)