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Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jun 11 – Sep 27, 2018
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on sampling:
The samples were taken from the biological phase of the study. Collecting, storage and handing over of the samples were the Study Director’s responsibility. The information concerning the samples was provided by the Study Director.
Duplicate samples from the freshly prepared test medium of the only test concentration and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the only test concentration and the control were collected at the end of the test (after 48 hours).
All samples were diluted by a factor of 2 with acetonitrile.
Additional samples of the control and the dilution solvent were taken at each sampling test start and test end without any sample treatment.
Details on test solutions:
The test item was not well soluble in test water. To avoid physical effects of undissolved test item on the daphnids, no concentration above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 104.7 mg of test item in 1047 mL test water. The stock solution was shaken overhead for 24 hours in the dark to dissolve as much test item as possible. Then, the undissolved test item was separated by filtration (0.45 μm cellulose acetate filter) and the filtrate was used as test medium.
The test media was prepared just before introduction of the daphnids (= start of the test).
Test organisms (species):
Daphnia magna
Details on test organisms:
Strain/Clone: Daphnia magna, clone 5;
Age: 1.5 to 19.25 hours old
Source: The daphnids introduced in the test were taken from in-house laboratory culture.
Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
250 mg/L
Test temperature:
20.4 to 20.9 °C
7.9 to 8.0
Dissolved oxygen:
≥ 8.6 mg O2/L in in all treatment groups
Nominal and measured concentrations:
100 mg/L (nominal)
< LOQ (measured)
Reference substance (positive control):
Potassium dichromate
Key result
48 h
Dose descriptor:
Effect conc.:
> 100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
48 h
Dose descriptor:
Effect conc.:
>= 100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Details on results:
After 48 hours of exposure no immobilisation of the test animals was observed in the control and the filtrate of the only tested test item concentration of nominal 100 mg test item/L.
Results with reference substance (positive control):
Results of the most recent Test with the Reference Item Potassium dichromate performed in January 2018
EC50 [mg/L]: 1.06 (24h) and 1.02(48 h)
Validity criteria fulfilled:
The toxic effect of the test item to Daphnia magna was assessed in a static limit test. The 48 -hour NOEC was determined to be at least the at the concentration of the filtrate of nominal 100 mg test item/L. The 48-hour EC50 was clearly higher than the filtrate of nominal 100 mg test item/L.

Description of key information

OECD 202: EC50 > 100 mg/L (nominal)

Key value for chemical safety assessment

Additional information