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Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Copper cyanide
EC Number:
208-883-6
EC Name:
Copper cyanide
Cas Number:
544-92-3
Molecular formula:
CCuN
IUPAC Name:
λ¹-copper(1+) iminomethanide

Method

Target gene:
Histidine (S. typhimurium) and Tryptophan (E. coli)
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Metabolic activation system:
rat liver S9 mix
Test concentrations with justification for top dose:
1st experiment
TA 98: 7.81, 15.63, 31.25, 62.5, 125, 250, 500 µg/plate (without S9 mix); 1.95, 3.91, 7.81, 15.63, 31.25, 62.5, 125 µg/plate (with S9 mix)
TA 100: 7.81, 15.63, 31.25, 62.5, 125, 250, 500 µg/plate (with and without S9 mix)
TA 1535 and TA 1537: 3.91, 7.81, 15.63, 31.25, 62.5, 125, 250 µg/plate (with and without S9 mix)
WP2 uvrA: 15.63, 31.25, 62.5, 125, 250, 500, 1000 µg/plate (with and without S9 mix)

2nd experiment
TA 98: 15.63, 31.25, 62.5, 125, 250, 500 µg/plate (without S9 mix); 7.81, 15.63, 31.25, 62.5, 125, 250, 500 µg/plate (with S9 mix)
TA 100: 31.25, 62.5, 125, 250, 500, 1000 µg/plate (with and without S9 mix)
TA 1535: 15.63, 31.25, 62.5, 125, 250, 500 µg/plate (with and without S9 mix)
TA 1537: 15.63, 31.25, 62.5, 125, 250, 500 µg/plate (without S9 mix); 7.81, 15.63, 31.25, 62.5, 125, 250 µg/plate (with S9 mix)
WP2 uvrA: 31.25, 62.5, 125, 250, 500, 1000 µg/plate (with and without S9 mix)
Vehicle / solvent:
1.5% Carboxymethylcellulose solution
Controls
Untreated negative controls:
yes
Positive controls:
yes
Positive control substance:
4-nitroquinoline-N-oxide
9-aminoacridine
2-nitrofluorene
sodium azide
benzo(a)pyrene
other: 2-aminoanthracene

Results and discussion

Test results
Key result
Species / strain:
other: S. typhimurium TA98, TA100, TA1535, TA1537 and E. coli WP2uvrA
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity, but tested up to precipitating concentrations
Untreated negative controls validity:
valid
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
The results of the bacterial reverse mutation assay indicated that, under the conditions of this study, Copper cyanide showed a negative response at the concentration ranges tested.