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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 AUG 2016 to 21 SEP 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: NF EN ISO 9439
Deviations:
no
GLP compliance:
yes
Remarks:
Accreditation Certificate : Fulfils the requirements of the standard NF EN ISO/CEI 17025 : 2005

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2-dimethyl-3-propyl imidazolium bis((trifluoromethyl)sulfonyl)amide
EC Number:
805-807-9
Cas Number:
169051-76-7
Molecular formula:
C10H15F6N3O4S2
IUPAC Name:
1,2-dimethyl-3-propyl imidazolium bis((trifluoromethyl)sulfonyl)amide
Test material form:
liquid
Details on test material:
Appearance : colorless oil
Composition: a base stock containing (C, 28.64; H, 3.61; F, 27.18; N, 10.02; O, 15.26; S, 15.29)
Molecular formula : C10H15F6N3O4S2
Molecular Weight : 419.12 g/mol
Purity >99% (purity determined by MNR)
Homogeneity : homogeneous
Specific details on test material used for the study:
Batch N° L16-0352

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, industrial (adaptation not specified)
Details on inoculum:
- Source of inoculum: Aeration tank of sewage treatment plant (Pierre-Bénite Rhône).
- Preparation of inoculum for exposure: pre-conditioning
- Initial cell/biomass concentration: 3.0x10E7 µorganisms/mL of inoculum
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
69.92 mg/L
Based on:
test mat.
Initial conc.:
69.96 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Test temperature: 22°C ± 1°C
- Air: about 30 mL/min
- Inoculum : 1.7x 10E4 µorganisms/mL in the final mixture

TEST SYSTEM
- Number of culture flasks : 2
- CO2 titration: BA(OH)2 titration with HCl (0.05 M)

SAMPLING
- Sampling frequency: Samples were taken on day 1, 3, 6, 10, 13, 17, 21, 24 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 flasks



Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Key result
Parameter:
% degradation (CO2 evolution)
Value:
5
Sampling time:
28 d
Remarks on result:
other: mean of two test flasks

Any other information on results incl. tables

CO2 release and biodegradability

PDMIM.NTF2                      

Reference substance

(Na benzoate)

Time (days) cumulated CO2* released (mg) biodegradability (%) Time (days) CO2 released*
 (mg)
Biodegrada-
bility 
Flask 1 Flask 2 Average
1 0.57 0.48 0.53 0.48 1 27.21 24.66
3 1.12 1.04 1.08 0.98 3 57.19 51.83
6 1.99 1.71 1.85 1.68 6 77.32 70.06
10 3.04 2.54 2.79 2.53 10 88.9 80.55
13 4.48 3.80 4.14 3.76 13 96.03 87.01
17 4.44 3.88 4.16 3.77 17 97.7 88.53
21 3.99 2.91 3.45 3.13 21 96.53 87.47
24 4.13 5.54 4.84 4.39 24 96.43 87.38
28 5.17 6.17 5.67 5.15 28 96.56 87.5

* CO2 released = total CO2 production - average CO2 production in blanks

PDMIM.NTF2 = 1-Propyl-2,3-Dimethylimidazolium bis(trifluoromethanesulfonyl)imide

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Biodegradability of Na benzoate (in 14 days): Limit 60%; value > 87% Total CO2 released in blank (mg CO2/L in 28 days): Limit < 70%, value 61 (flask 1) - 59 (flask 2) Difference of extremes of replicate value: limit < 20%, value 16 %
Interpretation of results:
not readily biodegradable
Conclusions:
In our experimental conditions: 1-Propyl-2,3-Dimethylimidazolium bis(trifluoromethanesulfonyl)imide is not easily biodegradable. Biodegradability of 1-Propyl-2,3-Dimethylimidazolium bis(trifluoromethanesulfonyl)imide is about 5 % within the 28-days period of the test.