1,2-dimethyl-3-propyl imidazolium bis((trifluoromethyl)sulfonyl)amide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 AUG 2016 to 21 SEP 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: NF EN ISO 9439

Deviations:

no

GLP compliance:

yes

Remarks:

Accreditation Certificate : Fulfils the requirements of the standard NF EN ISO/CEI 17025 : 2005

Test material

Test material information

Constituent 1

Reference substance name:

1,2-dimethyl-3-propyl imidazolium bis((trifluoromethyl)sulfonyl)amide

EC Number:

805-807-9

Cas Number:

169051-76-7

Molecular formula:

C10H15F6N3O4S2

IUPAC Name:

1,2-dimethyl-3-propyl imidazolium bis((trifluoromethyl)sulfonyl)amide

Test material form:

liquid

Details on test material:

Appearance : colorless oil
Composition: a base stock containing (C, 28.64; H, 3.61; F, 27.18; N, 10.02; O, 15.26; S, 15.29)
Molecular formula : C10H15F6N3O4S2
Molecular Weight : 419.12 g/mol
Purity >99% (purity determined by MNR)
Homogeneity : homogeneous

Specific details on test material used for the study:

Batch N° L16-0352

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, industrial (adaptation not specified)

Details on inoculum:

- Source of inoculum: Aeration tank of sewage treatment plant (Pierre-Bénite Rhône).
- Preparation of inoculum for exposure: pre-conditioning
- Initial cell/biomass concentration: 3.0x10E7 µorganisms/mL of inoculum

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

69.92 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

69.96 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 22°C ± 1°C
- Air: about 30 mL/min
- Inoculum : 1.7x 10E4 µorganisms/mL in the final mixture

TEST SYSTEM
- Number of culture flasks : 2
- CO2 titration: BA(OH)2 titration with HCl (0.05 M)

SAMPLING
- Sampling frequency: Samples were taken on day 1, 3, 6, 10, 13, 17, 21, 24 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 flasks

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

5

Sampling time:

28 d

Remarks on result:

other: mean of two test flasks

Any other information on results incl. tables

CO2 release and biodegradability

PDMIM.NTF2										Reference substance (Na benzoate)
Time (days)	cumulated CO2* released (mg)			biodegradability (%)						Time (days)	CO2 released*  (mg)	Biodegrada- bility
	Flask 1	Flask 2	Average
1	0.57	0.48	0.53	0.48						1	27.21	24.66
3	1.12	1.04	1.08	0.98						3	57.19	51.83
6	1.99	1.71	1.85	1.68						6	77.32	70.06
10	3.04	2.54	2.79	2.53						10	88.9	80.55
13	4.48	3.80	4.14	3.76						13	96.03	87.01
17	4.44	3.88	4.16	3.77						17	97.7	88.53
21	3.99	2.91	3.45	3.13						21	96.53	87.47
24	4.13	5.54	4.84	4.39						24	96.43	87.38
28	5.17	6.17	5.67	5.15						28	96.56	87.5

* CO₂ released = total CO2 production - average CO2 production in blanks

PDMIM.NTF2 = 1-Propyl-2,3-Dimethylimidazolium bis(trifluoromethanesulfonyl)imide

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Biodegradability of Na benzoate (in 14 days): Limit 60%; value > 87% Total CO2 released in blank (mg CO2/L in 28 days): Limit < 70%, value 61 (flask 1) - 59 (flask 2) Difference of extremes of replicate value: limit < 20%, value 16 %

Interpretation of results:

not readily biodegradable

Conclusions:

In our experimental conditions: 1-Propyl-2,3-Dimethylimidazolium bis(trifluoromethanesulfonyl)imide is not easily biodegradable. Biodegradability of 1-Propyl-2,3-Dimethylimidazolium bis(trifluoromethanesulfonyl)imide is about 5 % within the 28-days period of the test.