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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Single administration of seven concentrations with an application volume of 20 ml/kg to fasted rats (5 males and 5 females) via stomach tube with an observation period of 14 days
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Test material form:
solid: crystalline
Details on test material:
3-Aminocrotonsäuremethylester, solid

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
Fasted (no food 16 h before and 4 h after application)

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Lutrol (PEG 400)
Doses:
1300, 1500, 1800, 1900, 2000, 2500 and 3100 mg/kg with an application volume of 20 ml/kg
No. of animals per sex per dose:
10 ( 5 males and 5 females)
Control animals:
no

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 760 mg/kg bw
Based on:
test mat.
95% CL:
>= 1 610 - <= 1 910
Mortality:
All deaths occurred on the first day after administration.
Clinical signs:
other: No symptoms at dose 1300 mg/kg. All animals at dose 1500 mg/kg and above symptomatic (e.g. reduced general condition, cyanosis, ventral or lateral position, narcosis, dyspnea). Clinical signs occurred after 5 to 10 min after administration, on day 3 all
Gross pathology:
Bleedings in the mucous membrane of the stomach.
Other findings:
No abnormal findings seen at final necropsy

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The acute oral LD50 was 1760 mg/kg.
Executive summary:

The acute oral toxicity of methyl 3 -aminocrotonate was determined in fasted male and female rats after single administration of seven concentrations with an application volume of 20 ml/kg via stomach tube with an observation period of 14 d. The acute oral LD50 was 1760 mg/kg.