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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 March 1989 through 26 December 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1984
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Nectaryl :
- Identification - Nectaryl - LRG 1371
- Batch No. - AS 1241001
Analytical monitoring:
no
Vehicle:
yes
Remarks:
Ethanol as co-solvent, maximum concentration added 5.07 mg/L (range = 0 (absolute Control) and 5.07 mg/L)
Details on test solutions:
A total of 8 test solutions were prepared, a Solvent Control and a Blank Control system.

The test solutions and Solvent Control contained a maximum of 5.07 mg of Ethanol per litre as co-solvent.

Test solution concentrations (Nominal - in mg of Nectaryl per litre) : 0.30, 0.44, 0.66, 1.00, 1.50, 2.25, 3.38, 5.07 mg Nectaryl/L (concentration spacing factor of 1.5-times).

Test Substance Concn. Co-Solvent Concn.
(mg/L Nectaryl) (mg/L EtOH)

0 (Control) 0
0 (Solvent Control) 5.07
0.30 0.30
0.44 0.44
0.66 0.66
1.00 1.00
1.50 1.50
2.25 2.25
3.38 3.38
5.07 5.07

Test organisms (species):
Daphnia magna
Details on test organisms:
- Supplier : Société d'Elevage Piscicole Contrôlé
- Age : Between 6 and 24 hours
- Holding Conditions : Covered glass dishes of approximately 8 Liters in capacity, containing approximately 5 Litres of medium
Lighting during holding/breeding - photoperiod 07h30 to 19h30
Diet during breeding period - Scendesmus subspicatus algae
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
Temperature Range : 20.4 to 20.9 °C
pH:
pH Range : 7.62 to 7.86
Dissolved oxygen:
Dissolved Oxygen concentration in all Incubates at end of 48 hour study period : > 6 mg O2/L.
Nominal and measured concentrations:
The Nominal Test concentrations tested were : 0.30, 0.44, 0.66, 1.00, 1.50, 2.25, 3.38, 5.07 mg Nectaryl/L

A Solvent Control (containing 5.07 mg Ethanol/L) and a Blank Control (no Ethanol) were run in parallel
Details on test conditions:
- 20 daphnids per level, divided in to 4 replicates
- 5 daphnids per 10 mL (loading 1 daphnid per 2 mL)
- Exposure vessels - glass test tubes
- Exposure conducted in darkness
- pH, Dissolved Oxygen and Temperature recorded at Time 0 and at 48 hours
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.49 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence interval : 0.33 to 0.73 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
2.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The 48 hour EC50 deteremined for Nectaryl under the test conditions applied was 0.49 mg/L.

Validity criteria were met :
- The dissolved oxygen concentration was > 6 mg O2/L at the end of the 48 hhour incubation;
- 5% immobilisation was observed in the blank Control and 10% in the Solvent Control over the 48 hour exposure
- No trapping observed in the Controls

Immobilisation Observed :

Nominal Exposure Concn. % Immobility
(mg/L) 24 hours 48 hours

Control 0 5
Solvent Control 0 10
0.30 35 40
0.44 20 45
0.66 30 50
1.00 65 75
1.50 90 95
2.25 40 100
3.38 90 100
5.07 100 100

Validity criteria fulfilled:
yes
Conclusions:
The 48 hour EC50 for Daphnia exposed to Nectaryl was determined under the applied test conditions to be : 0.49 mg/L (95% confidence intervals : 0.33 to 0.73 mg/L).
Executive summary:

The 48 hour EC50 for Daphnia exposed to Nectaryl was determined under the applied test conditions to be : 0.49 mg/L (95% confidence intervals : 0.33 to 0.73 mg/L).

Description of key information

The 48 hour EC50 for Daphnia exposed to Nectaryl was determined under the applied test conditions to be : 0.49 mg/L (95% confidence intervals : 0.33 to 0.73 mg/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.49 mg/L

Additional information