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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Test material form:
liquid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Purity : 96.8%

Sampling and analysis

Analytical monitoring:
yes
Remarks:
at the begin, 24 and 48 h

Test solutions

Details on test solutions:
Juvenile daphnids were exposed to an aqueous test medium containing the test item at five nominal concentrations in a geometric series (2.0, 6.3, 20.0, 63.2, 200.0 microg/l).
A solvent control was prepared by adding 50 microl acetone to 500 ml of reconstituted water in order to obtain the same solvent concentation of the test solution (100 ul).
A control with reconstituted water only was also tested.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
ACCLIMATION
- Acclimation period:12 days
- Acclimation conditions: same as test

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Check for immobilisation 24 and 48 h after test initiation

Test conditions

Hardness:
2.5 mmol/L (250.0 mg/l as CaCO3)
Test temperature:
20 +/-2°C with a maximum deviation of +/-1°C during the individual study. 19.0 - 21.0°C.
pH:
7,32 - 7,72
Dissolved oxygen:
6.1 - 7.0 mg/l
Nominal and measured concentrations:
2.0, 6.3, 20.0, 63.2, 200.0 microg/l
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
5 µg/L
Nominal / measured:
meas. (geom. mean)
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
16 µg/L
Nominal / measured:
meas. (geom. mean)
Key result
Duration:
48 h
Dose descriptor:
IC50
Effect conc.:
9.5 µg/L
Nominal / measured:
meas. (geom. mean)
Remarks on result:
other: 7.5-10.5
Details on results:
The test item concentration was not stable during the test, the measured concentrations being around 55% and 20-40% of the nominal already short after being prepared (time 0 h and time 24 h, fresh solution rescpectively), while further decrease occurrred during the following day (to around 3-30 % of the nominal). Therefore the biological results are not referred to the nominal concentration but to the geometric mean of the measured concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The results, calculated based on the mean of the measured exposure concentrations were as follows:
48h NOEC: 5.0 microg/l
48h LOEC: 16.0 microg/l
48h IC50: 9.5 microg/l (95% confidence range: 7.5 - 10.5 microg/l)