1-Methyl-1-propylpyrrolidinium Bis(fluorosulfonyl)imide

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020-11-26 to 2021-02-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

On 03/12/2020, activated sludge was sampled near the exit of the aeration tank at Valence’s waste water treatment plant receiving predominantly domestic sewage. The sludge was settled and the liquid supernatant discarded. The concentrated sludge was then suspended in the mineral solution. These two steps were repeated twice.

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation:

O2 consumption

Key result

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

 No lag phase and biodegradation were highlighted.
 10-d window is not respected
 Percentage of biodegradation at 28 days: 0 %

Results with reference substance:

Based on a biodegradation rate higher than 70% in 14 days (experimental value: 79.6 and 78.0 % in 1 day) in the reference control, the test was carried out under good conditions.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Under the conditions of an experimental study performed according to the OECD 301F guideline under GLP conditions, the test item was found to be not readily biodegradable.

Executive summary:

The objective of this study was to assess the aerobic ready biodegradability of the test item N-PropylN-methylpyrrolidinium Bis(fluorosulfonyl)imide by measurement of manometric respirometry over a 28- day period according to the OECD 301F guideline (July 1992).

 

The test item, at 100 mg.L-1 , as the sole source of carbon and energy, was incubated in a buffer-mineral salts medium which was inoculated with a mixed population of micro-organisms. The consumption of oxygen was determined from the change in pressure in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThOD.

The study is valid since:

Based on a biodegradation rate higher than 60% in 14 days (experimental value: 67.3 and 69.5 % in 3 days) in the reference control, the test was carried out under good conditions. At the end of the test, measurements of Biological Oxygen Demand (BOD) in two replicates of the test item solution did not show a difference of more than 20% (experimental value: 0 %). The oxygen uptake of the control was not greater than 60 mg.L-1 in 28 days (experimental value: 15.7 and 18.6 mg O2.L-1 ). Based on a biodegradation rate higher than 25% in 14 days (experimental value: 39.6 % in 3 days) in the toxicity control, the test item was not toxic to micro-organisms. No significant Biological Oxygen Demand of the abiotic control over the test period was highlighted. There was therefore no release of oxygen. The degradation of test item was not abiotic. The pH value were in the range 6-8.5 in the test vessel.

 

Results are the following:

	N-Propyl-N-methylpyrrolidinium Bis(fluorosulfonyl)imide
Percentage of biodegradation at 28 days	0 %
Conclusion	Not ready biodegradable

Description of key information

Under the conditions of an experimental study performed according to the OECD 301F guideline under GLP conditions, the test item was found to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater