Reaction mass of (((1,3-phenylenebis(oxy))bis(ethane-2,1-diyl))bis(oxy))bis(ethane-2,1-diyl) bis(2-methylacrylate) and (1,3-phenylenebis(oxy))bis(propane-3,1-diyl) bis(2-methylacrylate) and 3-(3-(2-(2-(methacryloyloxy)ethoxy)ethoxy)phenoxy)propyl methacrylaterate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22th-24th October, 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2021

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of (((1,3-phenylenebis(oxy))bis(ethane-2,1-diyl))bis(oxy))bis(ethane-2,1-diyl) bis(2-methylacrylate) and (1,3-phenylenebis(oxy))bis(propane-3,1-diyl) bis(2-methylacrylate) and 3-(3-(2-(2-(methacryloyloxy)ethoxy)ethoxy)phenoxy)propyl methacrylaterate

EC Number:

944-271-1

Cas Number:

2305048-54-6

Molecular formula:

not applicable for multi-constituent.

IUPAC Name:

Reaction mass of (((1,3-phenylenebis(oxy))bis(ethane-2,1-diyl))bis(oxy))bis(ethane-2,1-diyl) bis(2-methylacrylate) and (1,3-phenylenebis(oxy))bis(propane-3,1-diyl) bis(2-methylacrylate) and 3-(3-(2-(2-(methacryloyloxy)ethoxy)ethoxy)phenoxy)propyl methacrylaterate

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 3M Maplewood, Lot# 653940
- Expiration date of the lot/batch: February 2020
- Purity test date: 26th February 2019
- Purity: 100% (UVCB)
- Physical state:yellowish to reddish liquid
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature in a dark, ventilated cabinet in the original
container.
- Stability under storage conditions: Stable
- Stability under test conditions: Stable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 (blank control) and 100 mg/L
- Sampling method: Water samples were taken from the approximate midpoint of each test concentration vessel upon initial exposure (0 hour), at the 24 hours period renewal (new solutions), the 24 hours old solution, and at the termination of the experiment (48 hour). Samples of fresh solutions were taken from the intermediate mixing vessels before division into replicates. For old solutions, pooled solutions each replicate were sampled. Test samples (5.0 mL) were taken from the exposure solutions and directly transferred to disposable glass vials containing methanol (to a final composition of 50/50 methanol/medium, v/v). Samples were then diluted into the calibration standard range with 50/50 methanol/medium, (v/v) prior to analysis. Three quality control samples, prepared in dilution water at nominal concentrations similar to the treatment level range, were also run during each sampling interval and analyzed with the study samples.
- Sample storage conditions before analysis: Analysis was run immediately after sampling. Subsamples of the test solutions were collected at each sampling interval and stored frozen as archive samples.
- Sample storage conditions before analysis: Analysis was run immediately after sampling. Subsamples of the test solutions were collected at each sampling interval and stored frozen as archive samples.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Primary stock solutions were made in fortified laboratory well water. The primary stock solution was prepared as a water-accomodated fraction (WAF) by adding the test substance directly to the total volume of dilution water in a glass beaker. Solution was clear and colorless with visible white globules on the surface of the solution and bottom of the test vessel. The solution was covered with plastic wrap and foil and mixed for 18 hours and 35 minutes using a magnetic stir plate and teflon coated stir bar. After overnight mixing, a 30 minute settling period was allowed, but visible undissolved test material remained. The WAF was siphoned from the middle of the mixing vessel and there was no noted undissolved material after mixing with a glass rod for 1 minute. The 100% WAF was used as the exposure solution. Exposure renewal solutions were made by the above procedure.
- Controls: Test medium without test substance, handled as above
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Age at study initiation: <24 hours.
- Source: Smithers culture
- Feeding during test : No
- Presence of ephippia: none
- Culture conditions: Adult daphnids were cultured in fortified laboratory well water, pH 7.6 - 8.1,
hardness 170-180 mg CaCO3/L, alkalinity 82-84 mg CaCO3/L, water temperatures 20 - 21°C, and
photoperiod was 16h light, 8 h dark (540 - 1000 lux). Daphnids in the cultures were fed once daily a suspension of green algae (Ankistrodesmus falcatus, 4X10^7 cell/mL) and YCT suspension (yeast, cereal leaves and flaked fish food).

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

180 CaCO3

Test temperature:

20-21 °C

pH:

8.0-8.2

Dissolved oxygen:

8.2-9.2 mg/L

Conductivity:

760 uS/cm

Nominal and measured concentrations:

Nominal: 0 (blank control) and 100 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass beaker covered with clear plastic
- Volume of solution: 200 mL
- Aeration: No
- Renewal rate of test solution: At 24 hours, daphnids transferred to fresh medium in new test vessels
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 25 daphnids/L ( 5 daphnids/ 200 mL)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water fortified with 192 mg/L NaHCO3, 120 mg/L CaSO4.
H2O, 120 mg/L MgSO4, 8.0 KCl
- Total organic carbon: 0.22 mg/L
- Particulate matter: < 1 mg/L
- Representative samples analyzed for pesticides, PCB's and toxic metals were not detected at
concentrations that are considered toxic in any of the water samples analyzed.
- Alkalinity: 82-84 mg CaCO3/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: semi-annually
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16h light, 8h dark
- Light intensity: 1000-1100 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization (
including mortality) at 24 and 48 hours.
VEHICLE CONTROL PERFORMED: No
RANGE-FINDING STUDY
- Test concentrations: 0.01, 0.1, 1.0 10 mg/L
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: No immobilization observed

Details on results:

- Mortality of control: No mortality of the control.
- Behavioral abnormalities: None reported
- Other adverse effects control: None reported
- Immobilisation of control: No immobilization of the control.

Results with reference substance (positive control):

24 hour EC50=1.9 mg/L (95% CI=1.7 to 2.2 mg/L). Reference test: 4th-5th September 2019 (47 days
before definitive test).

Reported statistics and error estimates:

CETIS v1.9 software (Tidepool Scientific Software, McKinleyville, CA)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

<10% mortality in controls (0%), Dissolved oxygen concentration was ≥ 3 mg/L during test period (DO≥8.2)

Conclusions:

48 hour EC50 >100 mg/L (OECD 202) in Daphnia magna

Executive summary:

The 48 hour EC50 to Daphnia magna was determined under semi-static conditions in a limit test according to OECD 202 guidelines. A water accommodated fraction with a nominal concentration of 100 mg/L, and a blank control were run using 5 animals per vessel in four replicates and no dispersant.
No immobilization nor mortalities were observed throughout the test. An EC50 >100 mg/L (nominal concentration) was determined.

The study was well-documented, followed an international standard method with analytical confirmation of dose and was GLP compliant. The study is considered reliable without restrictions. The results from this study are considered suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.