[No public or meaningful name is available]

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Due to the low aqueous solubility and complex nature of the test item, for the purposes of the study the test medium was prepared as a Water Accommodated Fraction (WAF) of the test item.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The test was carried out using juvenile rainbow trout (Oncorhynchus mykiss).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

15°C

pH:

pH at the beginning of the test: 8.0
pH at the end of the test: 7.8

Dissolved oxygen:

Dissolved oxygen at the beginning of the test: 9.9 mg O2/L
Dissolved oxygen at the end of the test: 8.8 mg O2/L

Nominal and measured concentrations:

Limit test concentration: 100 mg/L loading rate WAF

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed in fish exposed to 100mg/L loading rate WAF.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Oncorhynchus mykiss 96h LL50 is > 100mg/L loading rate of the Water Accommodated Fraction (WAF). The No Observed Effect Loading rate was 100 mg/L loading rate WAF.

Executive summary:

Short-term toxicity to fish of the test item was evaluated according to OECD guideline 203 (Fish, Acute Toxicity Test). Rainbow trout (Oncorhynchus mykiss) were exposed for 96 hours to the water accommodated fraction (WAF) of the test item at a single nominal loading rate of 100 mg/L. Exposure of rainbow trout to the test item gave LL50 values of greater than 100 mg/L loading rate WAF and a no observed effect loading rate (NOAELR) of 100 mg/L loading rate WAF. This results in the test item being unclassified for short-term toxicity to fish, according to GHS criteria.