2,2,3,3,4,4,5,5,6,6-decafluoro-6-trifluorovinyloxyhexanenitrile

Endpoint summary

Additional information

MV5CN is volatile and hydrolytically active. A half-life of 1.32 hours was measured at pH 7 and ambient temperature. To the extent that the substance remains in test medium, it would entirely hydrolyze to MV5CN amide during the test solution preparation. Therefore, in accordance with Chapter R.7B of the Guidance on information requirements, the hydrolysis product was tested directly.

In addition, studies were performed on MV5CN. Analytically determined concentrations were below detection limits in solution at all times during these tests. It is not known whether the test substance
was lost due to volatilization, hydrolysis, or some other mechanism.

All aquatic toxicity studies run with MV5CN amide were performed in accordance with internationally-accepted test guidelines and Good Laboratory Practice (GLP) standards. These results
are considered reliable without restrictions. The results with MV5CN amide are suitable for purposes of Risk Assessment, Classification & Labeling, and PBT Analysis. The studies run with MV5CN
are not considered reliable due to known or suspected loss of test material during the study period and should not be used for the purposes of Risk Assessment, Classification & Labeling, and PBT Analysis.
Results are reproduced below.

Species	Test Material	Endpoint	Concentration	Results parameter	Reliabiltiy (Klimisch)
Invertebrate
Daphnia magna	MV5CN amide	EC50	0.033 mg/L	immobilization	1
Daphnia magna	MV5CN	EC50	>0.5 mg/L	immobilization	4*
Daphnia Magna	MV5CN	EC50	100 mg/L	immobilization	3*
Algae
Pseudokirchneriella subcapitata	MV5CN amide	EC50 EC10	>2.5 mg/L 1.8 mg/L	growth rate	1

*Analytical measurements showed no detectable test substance in solutions during test. Method of removal is unknown (volatilization/hydrolysis etc.). Data not usable for classification purposes.