Benzoic acid, 3-amino-5-[[[2,3-bis(acetyloxy)propyl]amino]carbonyl]-2,4,6-triiodo-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Mar 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Test duration not in line with today's standard methods. However, since no effects (visual abnormalities and mortality) were recorded after 48 h no lethal effects are expected to occur after an additional of 48 h.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

rac-5-Amino-N-(2,3-diacetoxypropyl)-2,4,6-triiodoisophthalamic acid

EC Number:

602-891-7

Cas Number:

122731-59-3

Molecular formula:

C15 H15 I3 N2 O7

IUPAC Name:

rac-5-Amino-N-(2,3-diacetoxypropyl)-2,4,6-triiodoisophthalamic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
200 g of the test substance were dissolved in 1 L of tap water and treated in an ultrasonic bath for 10 min. This solution was added to 15 L of tap water. 290 mL 1 N NaOH was added to the solution to produce a pH value of 7.3. The resulting solution was further diluted with 3.71 L of tap water, to reach a nominal test concentration of 10 g/L.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Zierfischzüchterei Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 2.5 cm

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Feeding frequency during acclimation: twice a day
- Health during acclimation (any mortality observed): no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

21.4 - 21.8 °C

pH:

7.4 - 7.9

Dissolved oxygen:

Control: 7.5 - 8.1 mg O2/L
ZK 91248: 2.5 - 8.5 mg O2/L

Nominal and measured concentrations:

nominal: control, 10 g/L

Details on test conditions:

Ten fish were used tor the test concentration and ten for the control group, randomly distributed into blocks of five. Only fish in good health and free from any apparent malformation were used. Labelled 21 L glass aquaria were used as test vessels and filled with 20 L of the test solution and the dilution water (control group). The test started when the animals were added to the previously prepared test solution. The fish were exposed to the concentration of the test substance for a period of 48 hours under static conditions. The test temperature was in the range of 21 °C ± 1°C. The light/dark rhythm was adjusted to 12 hours/12 hours.

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of Tamip diacetate (ZK 91.248) in zebra fish is higher than 10 g/L

Executive summary:

The compound was studied in an acute toxicity test in the zebrafish Danio rerio. The test was conducted as a limit test with a concentration of 10 g/L over a period of 48 hours. No mortality of visuable abnormality was observed. The LC50 was higher than 10 g/L.