1,1'-Biphenyl, 4'-chloro-2-nitro-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study with analytical test item concentration verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Chlornitrobiphenyl
- Physical state: liquid
- Analytical purity: 95.5 %
- Lot/batch No.: 27997/111 Fr.5
- Storage condition of test material: not indicated

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Bio International B.V. Postbus 616; 6040 AP Roermond, Netherlands
- Age at study initiation: about 6 months
- Length at study initiation (length definition, mean, range and SD): 3.07 (2.6 - 3.4) cm
- Weight at study initiation (mean and range, SD): 0.17 g (0.10 - 0.30)
- Feeding during test
- Food type: Standard feed (TETRA MIN), on workdays generally additionally live artemia
- Amount: ad libitum


ACCLIMATION
- Conditions equivalent to housing

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: about 2.5 mM (about 250 mg/L CaCO3)
Acid capacity: about 5.5 mM

Test temperature:

22 °C

pH:

8.0 - 8.6

Dissolved oxygen:

7.6 - 8.9 mg/L

Nominal and measured concentrations:

nominal: 0, 0.0464, 0.1, 0.215, 0.464, 1.0, 2.15 and 4.64 mg/L
percentag of nominal at beginning: 25.4 - 47.9%
percentag of nominal after 96 h: 61.6 - 40.3%

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L fill volume (water depth about 17 cm)

- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.2 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated, char coal filtered
- Ca/Mg ratio: 9:1
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and symptoms


TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately 2.2
- Range finding study
- Test concentrations: 10 - 100 mg/L (no further data given)
- Results used to determine the conditions for the definitive study: yes

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.777 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)