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EC number: 211-720-1 | CAS number: 691-37-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Test start: 22 June 2004. Test completed: 14 December 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to GLP and guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- 4-methylpent-1-ene
- EC Number:
- 211-720-1
- EC Name:
- 4-methylpent-1-ene
- Cas Number:
- 691-37-2
- Molecular formula:
- C6H12
- IUPAC Name:
- 4-methylpent-1-ene
- Details on test material:
- - Name of test material (as cited in study report): 4-methyl-1-pentene
- Analytical purity: 98.36%
- Lot/batch No.: 3B24A
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories Japan Inc. (Atsugi Breeding Center)
- Age at study initiation: 8 female rats at 7 weeks of age for groups 1 and 2, and 8 female rats at 6 weeks of age for groups 3 and 4
- Weight at study initiation: 223-240 g
- Fasting period before study: The animals were fasted overnight (17-18 hours)
- Housing: The animals were housed in bracket-type mesh-floored metal cages (260W x 380D x 180H mm), two animals per cage during the quarantine and acclimation period, and individually after grouping
- Diet: The animals had free access to γ radiation-sterilized pellet diet CRF-1, Oriental Yeast Co., Ltd., in metal feeders
- Water: The animals had free access to Sapporo City tap water from an automatic water supply system
- Acclimation period: After receipt the animals were quarantined and acclimated: animals in group 1 until day 13 of acclimation, in group 2 until day 16 of acclimation, in group 3 until day 20 of acclimation and in group 4 until day 23 of acclimation
ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 3°C (actual range: 19-24°C)
- Humidity: 50 ± 20% (actual range: 45-75%
- Air changes: 10-15 times/hour
- Photoperiod: lighting for 12 hours (artificial light, 8:00-20:00)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: Group 1: 60 mg/L Group 2: 60 mg/L Group 3: 400 mg/L Group 4: 400 mg/L
- Justification for choice of vehicle: Since 4-methyl-1-pentene is insoluble in water, corn oil was selected as the vehicle in this study
- Lot No: V2T2225, Nacalai Tesque, Inc
DOSAGE PREPARATION: Refer to material and methods
CLASS METHOD:
- Rationale for the selection of the starting dose: Because there was no information of the toxicity of the test substance, the dose level in group 1 was set at 300 mg/kg in this study The next dose level was determined based on the mortality up to day 3 after administration. Because no deaths occurred in groups 1 or 2, the dose level in group 3 was set at 2000 mg/kg. Because no deaths occurred in group 3 either, the dose level in group 4 was also set at 2000 mg/kg. - Doses:
- Group 1: 300 mg/kg
Group 2: 300 mg/kg
Group 3: 2000 mg/kg
Group 4: 2000 mg/kg - No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: All animals were observed for mortality, appearance, behavior, etc. on the day of administration (day 0) continuously from immediately to 1 hour after administration, and then at 2 and 4 hours after administration. Animals were observed twice a day, in the morning and afternoon, from day 1 to day 13 after administration, and once in the morning on day 14 after administration
- Frequency of observations and weighing: All animals were weighed on days 0 (before dosing on the day of administration), 1, 3, 5, 7, 10 and 14 after administration
- Necropsy of survivors performed: After observation of external appearance on day 14 after administration, all animals were euthanized by ether anesthesia, and organs/tissues of the entire body were examined macroscopically - Statistics:
- Mean values and standard deviations were calculated for body weight, body weight gain and the rate of increase
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- No deaths occurred during the 14 days after administration either in the two 300 mg/kg groups (6 animals) or in the two 2000 mg/kg groups (6 animals). Mortality data is presented in Table 1.
- Clinical signs:
- other: No signs of toxicity were observed during the 14 days after administration either in the two 300 mg/kg groups (6 animals) or in the two 2000 mg/kg groups (6 animals) Results on clinical observation are shown in Table 2.
- Gross pathology:
- No abnormal findings were observed in in any animal either in the two 300 mg/kg groups (6 animals) or in the two 2000 mg/kg groups (6 animals). Necropsy findings are shown in Table 4.
Any other information on results incl. tables
Table 1: Mortality of female rats in acute oral toxicity test of 4-methyl-1-pentene
Group |
Number of dead animals |
Mortality |
|||||
Hours after administration |
Days after administration |
||||||
0 |
0.5 |
1 |
2 |
4 |
1-14 |
||
4-Methyl-1-Pentene 300 mg/kg |
0 |
0 |
0 |
0 |
0 |
0 |
0/6 |
4-Methyl-1-Pentene 2000 mg/kg |
0 |
0 |
0 |
0 |
0 |
0 |
0/6 |
Table 2: General appearance of female rats in acute oral toxicity test of 4-methyl-1-pentene
Group findings |
Hours after administration |
Days after administration |
||||
0 |
0.5 |
1 |
2 |
4 |
1-14 |
|
4-Methyl-1-Pentene 300 mg/kg Number of animals examined No abnormal findings |
6
6 |
6
6 |
6
6 |
6
6 |
6
6 |
6
6 |
4-Methyl-1-Pentene 2000 mg/kg Number of animals examined No abnormal findings |
6
6
|
6
6
|
6
6
|
6
6
|
6
6
|
6
6
|
Values are number of animals with findings
Table 3: Body weight changes of female rats in acute oral toxicity test of 4-methyl-1-pentene
Group |
Number of animals |
Body weight (g) on day after the administration |
Body weight gain 1-14 |
|||||||
0 |
1 |
3 |
5 |
7 |
10 |
14 |
(g) |
(%) |
||
4-Methyl-1-Pentene 300 mg/kg
|
6 |
206.5 3.7 |
224.3 10.1 |
237.7 7.1 |
241.7 12.5 |
250.3 8.2 |
256.3 7.4 |
261.2 8.4 |
54.7 6.6 |
26.465 3.099 |
4-Methyl-1-Pentene 2000 mg/kg
|
6 |
202.5 3.8 |
221.5 7.8 |
234.2 6.0 |
235.5 8.4 |
239.2 8.8 |
247.8 4.4 |
256.8 6.9 |
54.3 6.9 |
26.853 3.613 |
Table 4: Gross findings of female rats in acute oral toxicity test of 4-methyl-1-pentene
Findings |
4-Methyl-1-pentene (mg/kg) |
|
300 |
2000 |
|
Number of animals |
6 |
6 |
No abnormal findings |
6 |
6 |
Values are number of animals with findings
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral median lethal dose (LD50) of 4-methyl-1-pentene was >2000mg/kg and is therefore not classified in accordance with CLP Regulation (EC) no. 1272/2008
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