Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

In view of the difficulties associated with the evaluation of aquatic toxicity of poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed. An approach endorsed by several important regulatory authorities in the EU and elsewhere (ECETOC 1996 and OECD 2000), is to expose organisms to a Water Accommodated Fraction (WAF) of the test item in cases where the test item is a complex mixture and is poorly soluble in water and in the permitted auxiliary solvents and surfactants.

The substance tested, a brown liquid, is an UVCB containing a mixture of substances with a low water solubility. Water Accomodated Fractions were prepared by mixing the test substance with aqueous test medium. After mixing the test substance was allowed to settle, the aqueous phase was siphoned off through glass wool and dilutions of this medium were used in testing the substance under study. This method of preparation was selected to maximise the solubility of the test item under specific test conditions. The results were expressed either as a dilution, or as concentrations of the substance expressed in mg/L. Because a specific analytical method could not be developed due to the complexity of the test substance, a method based on turbidity of test solutions was developed.

This means that the dispersed fraction was quantified with this method. This aspecific method provides no information about stability of the test substance in the medium. Therefore, no samples were analysed at the end of the exposure but only initial concentrations were measured.