3,3',5,5'-tetra-tert-butylbiphenyl-2,2'-diol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, a solubiliser was used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3',5,5'-tetra-tert-butylbiphenyl-2,2'-diol

EC Number:

407-920-5

EC Name:

3,3',5,5'-tetra-tert-butylbiphenyl-2,2'-diol

Cas Number:

6390-69-8

Molecular formula:

C28 H42 O2

IUPAC Name:

3,3',5,5'-tetra-tert-butyl-[1,1'-biphenyl]-2,2'-diol

Details on test material:

- Name of test material (as cited in study report): CA 16-178
- Lot/batch No.: A 88103/Z1572R
- Purity: Lab. sample

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test solutions were prepared separately, a stock solution was not prepared.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): A slight deposit was observed at conc. 10-100 mg/L (nominal) after 72 h exposure.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): alkylphenol-polyglykol-ether
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 4 mg/L at highest test concentration

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 21 mm (16-26 mm)
- Weight at study initiation (mean and range, SD): 0.09 g (0.06-0.13 g)


ACCLIMATION
- Acclimation period: 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

186 mg CaCO3/L

Test temperature:

23 ± 1 °C

pH:

7.6 - 8.5

Dissolved oxygen:

96 - 101%

Nominal and measured concentrations:

Nominal test concentrations:
control, vehicle control 10, 18, 32, 58, 100 mg/L

Measured test substance concentrations:
at test start: 8.45, 13.49, 21.26, 45.45, 90.31 mg/l

at test termination: 7.95, 14.19, 27.30, 58.50, 81.92 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (36/22/25 cm)
- Fill volume: 15 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.06 g fish/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: fluorescent light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 24, 48 72 and 96 hours

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Symptoms of intoxication were not observed.