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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jun 2016 - Nov 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-methanesulfonyl-2-(trifluoromethyl)benzaldehyde
EC Number:
812-681-9
Cas Number:
1215310-75-0
Molecular formula:
C9H7F3O3S
IUPAC Name:
4-methanesulfonyl-2-(trifluoromethyl)benzaldehyde
Test material form:
solid: particulate/powder

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic, adapted
Details on inoculum:
The freshly obtained sludge was preconditioned to experimental conditions by continuous aeration until further treatment. The concentration of suspended solids was determined to be 3.4 g/L in the concentrated sludge. Before use, the sludge was allowed to settle (44 minutes) and the
supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium
Duration of test (contact time):
>= 0 - <= 28 d
Initial test substance concentration
Initial conc.:
28 mg/L
Based on:
formulation
Details on study design:
Test duration
28 days (last CO2 measurement on day 29).
During the test period, the test media were aerated and stirred continuously.
Test vessels
2 litre glass brown coloured bottles.
Milli-RO water
Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon.
Stock solutions of mineral components
A)8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli-RO water and made up to 1 litre, pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-RO water and made up to 1 litre.
C) 36.40 g CaCl2.2H2O dissolved in Milli-RO water and made up to 1 litre.
D) 0.25 g FeCl3.6H2O dissolved in Milli-RO water and made up to 1 litre.

Mineral medium
1 litre mineral medium contains: 10 mL of solution (A), 1 mL of solutions (B) to (D) and Milli-RO water.
Barium hydroxide 0.0125 M Ba(OH)2 (Boom, Meppel, The Netherlands), stored in a sealed vessel to prevent absorption of CO2 from
the air.
Synthetic air (CO2 < 1 ppm)1
A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was sparged through the
scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
Illumination
The test media were excluded from light.

Pre-incubation medium
The day before the start of the test (day -1) mineral components, Milli-RO water (ca. 80% of final volume) and inoculum (1% of final volume) were added to each bottle.
This mixture was aerated with synthetic air overnight to purge the system of CO2.
Type and number of bottles
Test suspension: containing test item and inoculum (2 bottles). Inoculum blank: containing only inoculum (2 bottles)
Positive control: containing reference item and inoculum (1 bottle).
Toxicity control: containing test item, reference item and inoculum (1 bottle).
Preparation At the start of the test (day 0), test and reference item were added to the bottles containing the microbial organisms and
mineral components.
The volumes of suspensions were made up to 2 litres with Milli-RO water, resulting in the mineral medium described
before.
Three CO2-absorbers (bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit air line of
each test bottle.
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
Key result
Parameter:
% degradation (CO2 evolution)
Value:
>= 0 - <= 3
Sampling time:
29 d
Details on results:
The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of QAW039-C2 (3% and 0%, based on ThCO2).
In the toxicity control, more than 25% biodegradation occurred within 14 days (30%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of QAW039-C2 (3% and 0%, based on ThCO2).
In the toxicity control, more than 25% biodegradation occurred within 14 days (30%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity.
Functioning of the test system was checked by testing the reference item sodium acetate, which showed a normal biodegradation curve.