4-Methanesulfonyl-2-(trifluoromethyl)benzaldehyde

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb - July 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-methanesulfonyl-2-(trifluoromethyl)benzaldehyde

EC Number:

812-681-9

Cas Number:

1215310-75-0

Molecular formula:

C9H7F3O3S

IUPAC Name:

4-methanesulfonyl-2-(trifluoromethyl)benzaldehyde

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The total exposure period was 48 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test.
3.0 mL from the approximate centre of the test vessels

Test solutions

Vehicle:

yes

Remarks:

Adjusted ISO medium

Details on test solutions:

QAW039-C2 1.0%, 10% and 100% of a SS prepared at a loading rate of 100 mg/L.
Controls Test medium without test item or other additives.

Preparation of test solutions started with a loading rate of 100 mg/L applying a 20-minute
treatment with ultrasonic waves followed by a 3-day period of magnetic stirring to ensure
maximum dissolution of the test item in medium. The obtained aqueous mixture was filtered
through a 0.45 μm membrane filter (RC55, Whatman) and the resulting Saturated Solution
(SS) was used as the highest test concentration. Lower test concentrations were prepared by
subsequent dilutions of the SS in test medium. All test solutions were clear and colorless at
the end of the preparation procedure.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (Crustacea, Cladocera) (Straus, 1820),
at least third generation, obtained by a cyclical
parthenogenesis under specified breeding conditions.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

18-22°C

pH:

6-9

Dissolved oxygen:

≥ 3 mg/L

Details on test conditions:

Test conditions remained within the limits prescribed by the study plan (pH: 6-9, not varying by
more than 1.5 units; oxygen: ≥3 mg/L at the end of the test).
The temperature continuously measured in a temperature control vessel varied between 20
and 21°C during the test, and complied with the requirements as laid down in the study plan
(18-22°C, constant within 2°C).

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h-EC50 for Daphnia magna exposed to QAW039-C2 was beyond the range tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.