Registration Dossier
Registration Dossier
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EC number: 209-057-8 | CAS number: 554-00-7
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
- Title:
- Soilless culture.
- Author:
- Steiner A.A.
- Year:
- 1 968
- Bibliographic source:
- Proceedings, Sixth Colloquium of the International Potash Institute, Florence, Italy, pp. 324-341
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- yes
- Remarks:
- : spacing factor should be = 3; replicates should be = 4
- Principles of method if other than guideline:
- The toxicity of 2,4-dichloroaniline to lettuce (Lactuca sativa) was determined in soil according to OECD TG 208 and in nutrient solution according to Steiner (1968). In the first case a static and in the latter a semistatic exposure was established. Both tests were carried out by two different Dutch institutes (RIVM and TNO).
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2,4-dichloroaniline
- EC Number:
- 209-057-8
- EC Name:
- 2,4-dichloroaniline
- Cas Number:
- 554-00-7
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 2,4-dichloroaniline
- Details on test material:
- - Name of test material (as cited in study report): 2,4-dichloroaniline
- Analytical purity: = 95%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- soil test: the concentration of the test compound was analyzed at the start and at the end of the test
nutrient solution: the concentration of the test compound was analyzed just before the next replacement of the test solution
Test substrate
- Vehicle:
- yes
Test organisms
- Species:
- Lactuca sativa
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: lettuce
- Variety: Ravel R2
Study design
- Test type:
- early seedling growth toxicity test
- Study type:
- other: study in climatic chamber
- Substrate type:
- other: natural soil and nutrient solution
- Limit test:
- no
Test conditions
- Test temperature:
- 21 ± 4°C
- pH:
- 7.5
- Moisture:
- humidity: 40 - 80%
- Details on test conditions:
- GROWTH CONDITIONS
- Photoperiod: 16:8 h light:dark
- Light intensity and quality: 6,500 lx - Nominal and measured concentrations:
- soil and nutrient solution
- nominal concentrations were spaced by a factor of 3.2
Results and discussion
Effect concentrationsopen allclose all
- Species:
- Lactuca sativa
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 32 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth (fresh weight of shoots)
- Remarks on result:
- other: 95% CL: 24-43; soil test at TNO
- Species:
- Lactuca sativa
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 24 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth (fresh weight of shoots)
- Remarks on result:
- other: 95% CL: 17-33; soil test at RIVM
- Species:
- Lactuca sativa
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 29 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth (fresh weight of shoots)
- Remarks on result:
- other: 95% CL: 24-36; soil test at TNO
- Species:
- Lactuca sativa
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 - < 32 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth (fresh weight of shoots)
- Remarks on result:
- other: soil test at RIVM
- Species:
- Lactuca sativa
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- 7 other: mg/l
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth (fresh weight of shoots)
- Remarks on result:
- other: 95% CL: 5.7-8.7; nutrient solution; test at TNO
- Species:
- Lactuca sativa
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 6.9 other: mg/l
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- growth (fresh weight of shoots)
- Remarks on result:
- other: 95% CL: 3.4-14; nutreint solution, test at RIVM
Applicant's summary and conclusion
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