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EC number: 289-296-2 | CAS number: 87061-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 March 2017 to 05 April 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- Concentrations: 100. 320 and 1000 mg/L
Samples were not stored prior to analysis - Vehicle:
- no
- Details on test solutions:
- The test item was dispersed directly in water.
Nominal amounts of test item (50, 160 and 500 mg (five replicates of each)) were each separately dispersed in approximately 200 mL of deionized reverse osmosis water and subjected to ultrasonication for approximately 15 minutes followed by magnetic stirring for 24 hours, at temperatures between 20 C and 21 C, in order to maximize the dissolved test item concentration. All test vessels were shielded from the light during mixing. Synthetic sewage (16 mL), activated sewage sludge (250 mL) and water were added to a final volume of 500 mL to give the required concentrations of 100, 320 and 1000 mg/L (five replicates of each).
The pH of the test item dispersions were measured after stirring using Hach HQ40d Flexi handheld meter (see Table 6) and adjusted to between pH 7.0 to pH 8.0 if necessary.
As it was not a requirement of the Test Guidelines, no analysis was conducted to determine the homogeneity, concentration or stability of the test item formulation. This is an exception with regard to GLP and has been reflected in the GLP compliance statement.
The control group was maintained under identical conditions but not exposed to the test item. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A mixed population of activated sewage sludge micro-organisms was obtained on
03 March 2017 for the range-finding test and on 04 April 2017 for the definitive test from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK which treats predominantly domestic sewage. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not applicable
- Test temperature:
- between 20 and 21°C
- pH:
- 6.4 -8.4 : after sturring and prior to addition of inoculum
7.-3 -7.4 at end of exposure period - Dissolved oxygen:
- 5.5 - 7.0 mg O2/L
- Salinity:
- Not applicable as freshwater study
- Nominal and measured concentrations:
- Range-finding study: 10, 100 and 1000 mg/L
Main study: 100, 320 and 1000 mg/L - Details on test conditions:
- Preparation of Test System
At time "0" 16 mL of synthetic sewage was diluted to 250 mL with water and 250 mL of inoculum added in a 500 mL conical flask (first control). The mixture was aerated with clean, oil-free compressed air via narrow bore glass tubes at a rate of 0.5 to 1.0 liter per minute. Thereafter, at 15 minute intervals the procedure was repeated for the second control followed by the reference item vessels with appropriate amounts of the reference item being added. Two additional control vessels were then prepared prior to the test item vessels were prepared as described in Section 3.3.2.1. Finally two further control vessels were prepared.
The test was conducted under normal laboratory lighting in a temperature controlled room at measured temperatures of between 20 and 21°C. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- No statistically significant toxic effects were shown at the test concentration of 100 mg/L, however statistically significantly toxic effects (P < 0.05) were shown at the test concentrations of 320 and 1000 mg/L. Based on these results the No Observed Effect Concentration (NOEC) after 3 hours exposure was 100 mg/L.
In some instances, the initial and final dissolved oxygen concentrations were outside those recommended in the test guidelines (7 mg O2/L and 2 mg O2/L respectively). This was considered to have had no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace.
The dissolved oxygen concentrations after 30 minutes contact time in all vessels were above 60% of the dissolved oxygen saturation level of 8.9 mg O2/L. - Results with reference substance (positive control):
- EC50: 8.9 mg/L (3 hours)
- Validity criteria fulfilled:
- yes
- Executive summary:
Introduction
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)".
Methods
Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 100, 320 and 1000 mg/L (5 replicates) for a period of 3 hours at measured temperatures of between 20 and 21°C with the addition of a synthetic sewage as a respiratory substrate.
The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5-dichlorophenol.
Results
The effect of the test item on the respiration of activated sewage sludge gave a No Observed Effect Concentration (NOEC) after 3 hours exposure of 100 mg/L.
The reference item gave a 3-Hour EC50value of 8.9 mg/L, 95% confidence limits 7.2 to 11 mg/L.
Reference
Range-Finding Test
The dissolved oxygen concentrations after 30 minutes contact time in all vessels were above 60% of the dissolved oxygen saturation level of 8.9 mg O2/L.
No statistically significant toxic effects were shown at the test concentrations of 10 and 100 mg/L, however statistically significant toxic effects were shown at the test concentration of 1000 mg/L.
Based on these results and after discussion with the Sponsor, it was considered necessary to perform a definitive test in order to obtain a NOEC for the test item. Based on this information test concentrations of 100, 320 and 1000 mg/L were selected for the definitive test.
Validation Criteria
The coefficient of variation of oxygen uptake in the control vessels was 6.90% and the specific respiration rate of the controls was 25.83 mg oxygen per gram dry weight of sludge per hour. The validation criteria have therefore been satisfied.
The validation criterion for the reference item EC50value was also satisfied
Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Definitive Test
Nominal |
Initial O2 |
Measurement Period |
Final O2Reading |
O2Consumption Rates |
% Inhibition |
|
Control |
R1 |
5.0 |
5 |
1.9 |
37.20 |
- |
R2 |
5.4 |
6 |
1.8 |
36.00 |
- |
|
R3 |
5.1 |
5 |
1.8 |
39.60 |
- |
|
R4 |
4.9 |
5 |
1.8 |
37.20 |
- |
|
R5 |
5.2 |
4 |
2.3 |
43.50 |
- |
|
R6 |
6.0 |
6 |
2.1 |
39.00 |
- |
|
Test Item |
100 R1 |
2.6 |
1 |
1.9 |
42.00 |
[8] |
100 R2 |
4.7 |
4 |
2.2 |
37.50 |
3 |
|
100 R3 |
4.3 |
4 |
1.9 |
36.00 |
7 |
|
100 R4 |
3.9 |
3 |
2.0 |
38.00 |
2 |
|
100 R5 |
4.0 |
3 |
2.1 |
38.00 |
2 |
|
320 R1 |
4.1 |
4 |
2.0 |
31.50 |
19 |
|
320 R2 |
4.5 |
4 |
2.4 |
31.50 |
19 |
|
320 R3 |
4.7 |
5 |
2.0 |
32.40 |
16 |
|
320 R4 |
4.7 |
6 |
1.7 |
30.00 |
23 |
|
320 R5 |
5.4 |
7 |
1.9 |
30.00 |
23 |
Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Definitive Test
Nominal |
Initial O2 |
Measurement Period |
Final O2Reading (mg O2/L) |
O2Consumption Rates |
% Inhibition |
|
Test Item |
1000R1 |
7.5 |
10 |
6.1 |
8.40 |
78 |
1000 R2 |
7.8 |
10 |
6.5 |
7.80 |
80 |
|
1000 R3 |
7.9 |
10 |
6.4 |
9.00 |
77 |
|
1000 R4 |
8.0 |
10 |
6.5 |
9.00 |
77 |
|
1000 R5 |
8.0 |
10 |
6.4 |
9.60 |
75 |
|
3,5-dichlorophenol |
3.2 |
5.8 |
7 |
2.1 |
31.70 |
18 |
10 |
7.0 |
10 |
4.1 |
17.40 |
55 |
|
32 |
7.8 |
10 |
7.0 |
4.80 |
88 |
R1– R6= Replicates 1 to 6
[Increase in respiration rate as compared to controls]
R1– R5= Replicates 1 to 5
Description of key information
A single high quality study, conducted under GLP conditions to OECD Guideline 209 was vailable to assess the substance's inhibitory effects on the respiration of activated sewage sludge. No statistically significant toxic effects were shown at the test concentration of 100 mg/L, however statistically significantly toxic effects were shown at the test concentrations of 320 and 1000 mg/L. Based on these results the No Observed Effect Concentration (NOEC) after 3 hours exposure was 100 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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