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EC number: 201-185-2 | CAS number: 79-20-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09.02.1988 - 16.02.1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- yes
- Remarks:
- QAU statement available
Test material
- Reference substance name:
- Methyl acetate
- EC Number:
- 201-185-2
- EC Name:
- Methyl acetate
- Cas Number:
- 79-20-9
- Molecular formula:
- C3H6O2
- IUPAC Name:
- methyl acetate
- Details on test material:
- - Name of test material (as cited in study report): Methylacetat
- Substance type: liquid
- Physical state: clear and colourless
- Analytical purity: 99.9% (w/w)
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: HOECHST AG, Kastengrund, conventional breeding
- Age at study initiation: ca. 3 - 5 months
- Weight at study initiation: 2.1 - 2.6 kg
- Housing: in fully conditioned rooms in single cages (batteries)
- Diet: Altromin 2123 Haltungsdiät - Kaninchen, ad libitum plus hay
- Water: deionised and dechloronosed water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 3°C
- Humidity (%): 50 +/- 20 %
- Photoperiod: 12 hours dark / 12 hours light
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the untreated right eye served as control
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied (volume or weight with unit): 0.1 ml applied into the left eye
- Concentration (if solution): pure test substance - Duration of treatment / exposure:
- evaluation after 1, 24, 48 and 72 hours after application
- Observation period (in vivo):
- 3 days plus an additional examination after 7 days
- Number of animals or in vitro replicates:
- 3 rabbits
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): treated eyes were washed with a sodium chloride solution on each examination date
- Time after start of exposure: 24 hours after application and on each examination date if the treates eyes still showed secretion
SCORING SYSTEM: accordin to guideline
TOOL USED TO ASSESS SCORE: fluorescein
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: mean values of the examinations after 24, 48 and 72 hours
- Score:
- 1.3
- Max. score:
- 1.7
- Reversibility:
- fully reversible within: 7 days after application
- Remarks on result:
- other: see table 1 for scores per each animal
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after appliocation
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- other: 48 and 72h
- Score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: mean values of the examinations after 24, 48 and 72 hours
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Remarks on result:
- other: see table 1 for scores per each animal
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: mean values of the examinations after 24, 48 and 72 hours
- Score:
- 2.7
- Max. score:
- 3
- Reversibility:
- fully reversible within: 7 days after application
- Remarks on result:
- other: At 24 and 72 hours after application, conjunctivae of the animals showed white discoloration, bleedings and partly ablations. See table 1 for scores per each animal
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 3
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 3
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 48/72h
- Score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 3
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: mean values of the examinations after 24, 48 and 72 hours
- Score:
- 1.8
- Max. score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Remarks on result:
- other: see table 1 for scores per each animal
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48 h
- Score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 3
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- other: 48/72h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- 3
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 48 h
- Score:
- 2
- Reversibility:
- fully reversible within: 7 days after application
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 1
- Reversibility:
- fully reversible within: 7 days after application
Any other information on results incl. tables
Table 1: Irritant response data for each animal at each observation time up to removal of each animal from the test
Score at time point / Reversibility |
Cornea |
Iris |
Conjunctivae |
Chemosis |
Max. score: 1 |
Max. score: 1 |
Max. score: 3 |
Max. score: 4 |
|
60 min |
1/1/1 |
1/1/1 |
2/2/1 |
4/4/3 |
24 h |
1/1/1 |
1/1/1 |
3/3/3 |
2/3/3 |
48 h |
1/2/1 |
1/1/1 |
3/2/3 |
2/1/2 |
72 h |
1/2/2 |
1/1/1 |
2/2/3 |
1/1/1 |
Average 24h, 48h, 72h |
1.3 |
1.0 |
2.7 |
1.8 |
Maximum average score |
1.7 |
1.0 |
3.0 |
2.0 |
Reversibility*) |
c |
c |
c |
c |
Average time (unit) for reversion |
7 days |
7 days |
7 days |
7 days |
*) Reversibility: c. = completely reversible; n.c. = not completely reversible; n. = not reversible
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based on the results, methyl acetate is classified as irritant (Xi) and has to be labelled with R36 (irritant to eyes).
- Executive summary:
Methyl acetate was applied to the rabbit's eye.The test concentration was 0.1 ml test substance per eye. Effects were examined after 1, 24, 48 and 72 hours after application. Due to the fact that effects were still observed 72 hours after application, an additional examination took place 7 days after application. At this time point, no effects were observed anymore. Based on the results, methyl acetate has to be classified as irritant to eyes.
In-vitro testing is not required.
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